The EDUCATE environment provides links to a number of commercial and non-commercial resources relating to flow cytometry and antibody-based techniques. You can link directly to the suppliers' websites.
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VivaFix™ Cell Viability Assays enable researchers to accurately discriminate between live and dead cells in fixed or unfixed samples by cell staining prior to flow cytometry, cell sorting and microscopy.
Bio-Rad offers a broad range of easy-to-use and versatile cell viability assays for assessing the cell health of fixed and unfixed cells in flow cytometry and cell sorting experiments.
We offer complete solutions for easy, sensitive determination of cell viability, cell vitality, and compound cytotoxicity. These fluorescence-based Invitrogen LIVE/DEAD™ assays can be used to examine animal cells, bacteria, yeast, and fungi. Specific LIVE/DEAD™ assays can be used for flow cytometry, microscopy, or microplate formats.
The ReadiDrop™ 7-aminoactinomycin D (7-AAD) cell viability assay is designed as a ready-to-use 7-AAD solution, which binds double-stranded DNA with a high-affinity intercalation between GC base pairs.
The easy-to-use cell viability assay range offers a choice of assessing the cell health of fixed and unfixed cells and the versatility to measure the viability of cells in Flow Cytometry, Microscopy and ELISA.
Commonly used in flow cytometry experiments for excluding dead cells, the propidium iodide solution enters a compromised cell and binds to double-stranded DNA/RNA by intercalating between base pairs.
Apoptosis and cell viability
Detection of apoptosis and cell viability by flow cytometry
While some assays utilize antibodies to study cell health, proliferation, cell cycle or apoptosis, other types of experiments can rely on non-antibody based methods of assessment, often called non-antibody chemical probes. These are reagents that localize to an organelle or indicate health based on chemical characteristics like hydrophobicity, charge, size and enzymatic activation.
It is critical to understand the degree of cell death in any flow cytometry assay and exclude those cells from the analysis.
The Viobility™ Fixable Dyes allow the discrimination between live and apoptotic or dead cells by flow cytometry. The dyes are suitable for both fixed and unfixed cells. Following reagents are available, addressing different fluorescent channels: