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Caspase Assays for Flow Cytometry

A distinctive feature of the early stages of apoptosis is the activation of caspase enzymes, which participate in the cleavage of protein substrates and in the subsequent disassembly of the cell. We provide a series of caspase assays that allow the simple detection of active caspases in living cells by flow cytometry.

Detecting Apoptosis

Apoptosis, a programmed cell death mechanism, is an essential process in embryogenesis and development. The process is also critical for ensuring an organism’s health by removing aberrant or damaged cells.

Apoptosis, or programmed cell death, is an essential process that ensures an organism’s health by eliminating damaged or aberrant cells. 

Apoptosis, or programmed cell death, is an essential process by which multi-cellular organisms ensure that critically damaged or mutated cells are removed. The process is regulated by a complex interplay of proteins and, until reaching “the point of no return”, can be halted or reversed at any point.

Apoptosis is a highly regulated process of programmed cell death which, unlike necrosis, does not promote inflammatory responses and is beneficial to an organism. 

MitoPT™ JC-1, TMRE & TMRM Kits​ Overview

• Choice – MitoPT™ JC-1, TMRE & TMRM Kits
• Quick & easy staining method clearly differentiates non-apoptotic & apoptotic cells
• MitoPT™ TMRE & TMRM Kits - ideal for single cell multi-parametric analyses
• Suitable for flow cytometry, fluorescence microscopy or a plate reader

Apoptosis Assays for Flow Cytometry

Apoptosis, a form of programed cell death, is directed by activated caspases. Caspases are cysteine proteases that can cleave other proteins, and upon activation, create a positive-feedback cascade, which ensures the cell will undergo apoptosis.

Apoptosis and cell viability

Detection of apoptosis and cell viability by flow cytometry

Apoptosis, a form of programed cell death, is directed by activated caspases. Caspases are cysteine proteases that can cleave other proteins, and upon activation, create a positive-feedback cascade, which ensures the cell will undergo apoptosis.