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Apoptosis, or programmed cell death, is an essential process by which multi-cellular organisms ensure that critically damaged or mutated cells are removed. The process is regulated by a complex interplay of proteins and, until reaching “the point of no return”, can be halted or reversed at any point.

Apoptosis, a form of programed cell death, is directed by activated caspases. Caspases are cysteine proteases that can cleave other proteins, and upon activation, create a positive-feedback cascade, which ensures the cell will undergo apoptosis.

Apoptosis is a highly regulated process of programmed cell death which, unlike necrosis, does not promote inflammatory responses and is beneficial to an organism. 

While some assays utilize antibodies to study cell health, proliferation, cell cycle or apoptosis, other types of experiments can rely on non-antibody based methods of assessment, often called non-antibody chemical probes. These are reagents that localize to an organelle or indicate health based on chemical characteristics like hydrophobicity, charge, size and enzymatic activation.

Apoptosis, or programmed cell death, is an essential process that ensures an organism’s health by eliminating damaged or aberrant cells. 

MitoPT™ JC-1, TMRE & TMRM Kits​ Overview

• Choice – MitoPT™ JC-1, TMRE & TMRM Kits
• Quick & easy staining method clearly differentiates non-apoptotic & apoptotic cells
• MitoPT™ TMRE & TMRM Kits - ideal for single cell multi-parametric analyses
• Suitable for flow cytometry, fluorescence microscopy or a plate reader

Apoptosis and cell viability

Detection of apoptosis and cell viability by flow cytometry

Detecting Apoptosis

Apoptosis, a programmed cell death mechanism, is an essential process in embryogenesis and development. The process is also critical for ensuring an organism’s health by removing aberrant or damaged cells.

Apoptosis, a form of programed cell death, is directed by activated caspases. Caspases are cysteine proteases that can cleave other proteins, and upon activation, create a positive-feedback cascade, which ensures the cell will undergo apoptosis.

Cathepsin Kits Overview 

• Quick and easy methods
• Analyze whole live suspension and adherent cells
• Assay based on fluorescent substrates
• Suitable for flow cytometry, fluorescence microscopy, or a plate reader