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Detection of apoptosis and cell viability by flow cytometry
Plasma membrane binding dyes for the analysis of apoptosis
Fixable protein-binding dyes for the analysis of apoptotic and dead cells
Non-fixable DNA dyes for the analysis of dead cells
Dead cells often give false positive results, as they tend to bind non-specifically to many reagents. Therefore, removing dead cells from your flow cytometry data is a critical step to ensure accurate results and analysis. Furthermore, multiparametric flow cytometry also allows for simultaneous analysis of the mechanisms of apoptosis.
