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Multicolor flow cytometry is the analysis of multiple fluorescent parameters in one sample. Building large flow cytometry panels can be daunting because each additional fluorophore you add to your panel has the potential to influence another fluorophore. 

Why fluorophores need compensating and how to avoid unnecessary compensation

Fluorophores accept light at a specific wavelength and re-emit it at a higher wavelength, giving each fluorophore an excitation and emission wavelength. Although there is a peak or maximal emission the range is often several hundred nanometers.

Watch our educational webinars to discover how recombinant antibodies can be generated with high specificity and high affinity to address challenges in antibody assay development and improve your bioanalytical and anti-drug antibody assays.

Learn about the details of how to setup a compensation workflow on a digital instrument such as the MACSQuant Analyzer, including the use of the MACSQuantify™ Software for automated compensation. Join Dr. Frank Hardung and his webinar on the compensation of spectral overlap in flow cytometry.

Join Dr. Frank Hardung and his webinar on sample preparation for flow cytometry – on tissue preparation, tissue dissociation, and sample clearance.

The quality of Flow Cytometry data is dependent on the quality of your cells. Understanding the best method of cell preparation, staining protocols and cell analysis can be crucial to obtaining valid data. 

Join Dr. Frank Hardung and his webinar on easy preparation of cell samples, on magnetic labeling and separation of cells, and on quality control of cell separations. Optimizing these basic steps is a prerequisite to successful cell analysis.

Apoptosis, or programmed cell death, is an essential process that ensures an organism’s health by eliminating damaged or aberrant cells. 

Join Frank Hardung and his webinar on rare cell analysis in flow cytometry, its general approaches, and proper controls.

Apoptosis is a highly regulated process of programmed cell death which, unlike necrosis, does not promote inflammatory responses and is beneficial to an organism.