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This advanced class discusses flow cytometry  sample preparation and the process of experimental design to ensure a successful flow cytometry experiment.

Flow Cytometry is a powerful tool for analyzing the physical and chemical properties of cells and other components such as nuclei and chromosomes. However, if you are a Flow Cytometry beginner, the whole process can be quite confusing.

Introduction

Tutorials for building multicolor flow cytometry panels always highlight the importance of antigen density - But why is it so important?

Antibodies are used to detect and quantify antigens in techniques such as flow cytometry, ELISA, western blotting, immunohistochemistry and lateral flow.

We know how challenging designing multi-color Flow Cytometry panels can be at times. That is why we have developed a set of tips to help you. Topics covered include: 

This on-demand webinar brings together a unique combination of content from two speakers with more than 50 years of experience in flow cytometry and antibody labeling. 

This webinar discusses how to overcome problems associated with using secondary antibodies in your immunoassay, including:

Flow cytometry is a powerful technique widely used in biological research, but if you are just beginning your flow journey, it can be hard to know where to start. 

This guide provides a general overview of each fluorochrome in our range including their properties, excitation and emission wavelengths and common applications.

Why Is Antibody Titration Important?

To optimize staining in flow cytometry antibody titration is recommended. Whilst antibodies will bind to high affinity targets on a cell, if they are present in excess they will also bind to low affinity targets.