The EDUCATE environment provides links to a number of commercial and non-commercial resources relating to flow cytometry and antibody-based techniques. You can link directly to the suppliers' websites.
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Flow Cytometry is a powerful tool for analyzing the physical and chemical properties of cells and other components such as nuclei and chromosomes. However, if you are a Flow Cytometry beginner, the whole process can be quite confusing.
Antibodies are used to detect and quantify antigens in techniques such as flow cytometry, ELISA, western blotting, immunohistochemistry and lateral flow.
Flow cytometry analysis typically begins with creating gates to distinguish cells of interest. This process of gating can appear quite random to a flow cytometry novice but it is in fact the most important part of flow cytometry analysis.
The flow cytometry protocols below provide detailed procedures for the treatment and staining of cells prior to using a flow cytometer.
This webinar discusses how to overcome problems associated with using secondary antibodies in your immunoassay, including:
Antibodies are widely employed in the quantification of antigens in complex biological samples. Using techniques such as Western blotting, ELISA, and immunohistochemistry researchers are able to measure a single antigen, or perhaps a limited number of antigens, in each sample. In the post-genomics era, advances in multiplex immunoassay technologies now allow scores or even hundreds of antigens to be measured simultaneously.
This advanced class discusses flow cytometry sample preparation and the process of experimental design to ensure a successful flow cytometry experiment.
This on-demand webinar brings together a unique combination of content from two speakers with more than 50 years of experience in flow cytometry and antibody labeling.
Flow cytometry protocols and staining procedures vary depending on whether the antigen to be detected is located on the cell surface or intracellular.
Flow cytometry is an invaluable technology used in a wide variety of biological research.