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Introduction to Flow Cytometry – Basics Guide

Flow cytometry is an invaluable technology used in a wide variety of biological research.

Antibodies are widely employed in the quantification of antigens in complex biological samples. Using techniques such as Western blotting, ELISA, and immunohistochemistry researchers are able to measure a single antigen, or perhaps a limited number of antigens, in each sample. In the post-genomics era, advances in multiplex immunoassay technologies now allow scores or even hundreds of antigens to be measured simultaneously.

This webinar discusses how to overcome problems associated with using secondary antibodies in your immunoassay, including:

Optimization of intracellular flow cytometry staining results with Leucoperm

Flow cytometry protocols and staining procedures vary depending on whether the antigen to be detected is located on the cell surface or intracellular.

Antibodies are used to detect and quantify antigens in techniques such as flow cytometry, ELISA, western blotting, immunohistochemistry and lateral flow.

Flow Cytometry is a powerful tool for analyzing the physical and chemical properties of cells and other components such as nuclei and chromosomes. However, if you are a Flow Cytometry beginner, the whole process can be quite confusing.

This advanced class discusses flow cytometry  sample preparation and the process of experimental design to ensure a successful flow cytometry experiment.

Flow cytometry analysis typically begins with creating gates to distinguish cells of interest. This process of gating can appear quite random to a flow cytometry novice but it is in fact the most important part of flow cytometry analysis. 

Direct and indirect staining, staining of intracellular antigens, permeabilization and cell preparation protocols

The flow cytometry protocols below provide detailed procedures for the treatment and staining of cells prior to using a flow cytometer.

This on-demand webinar brings together a unique combination of content from two speakers with more than 50 years of experience in flow cytometry and antibody labeling.