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Beyond antibody reagents, flow cytometry requires the right types of buffers for optimal staining. This convenient list separates out flow cytometry applications by their intended target. It also indicates which buffers are best-suited to your task for surface or intracellular staining and the protocols necessary for each.

True-Stain Monocyte Blocker™ has been formulated to block non-specific binding of PE/Dazzle™ 594, PE/Cy5, PE/Cy7, APC/Cy7 and APC/Fire™ 750 commonly seen on monocytes and macrophages.  The blocker is most effective when doing surface staining on live cells prior to the fixation step in a staining protocol.    Learn more.

In addition to the broad range of flow cytometry validated antibodies we offer for immunology, cancer and veterinary science, we also have supporting reagents that will optimize staining and sample preparation.

Miltenyi Biotec offers a huge range of fully validated antibodies suitable for multiple applications, such as flow cytometry, microscopy, and functional assays. The MACS™ Antibody Portfolio guarantees high performance consistency, with the majority manufactured using our innovative and proprietary recombinant antibody technology for unrivalled lot-to-lot reproducibility and standardization.  

Human Fc receptors are expressed on a variety of immune cell types including monocytes, macrophages, B cells, granulocytes and dendritic cells. Cells that express Fc receptors can give false positive immunofluorescent staining due to the Fc receptors binding of Ig. Human SeroBlock is designed to prevent such non-specific staining without interfering with appropriate target staining. Human SeroBlock is compatible with use of anti-human antibodies targeting Fc receptors in flow cytometry.

Mouse Seroblock FcR is a rat antibody that recognises mouse CD16 and CD32, cell surface proteins also known as FcRγIII and FcR&gamma:II, respectively. The function of these proteins is to bind IgG molecules via their Fc regions as part of the adaptive immune response.

Clinical flow cytometry

Diagnostic and quality-control reagents for cell manufacturing

Multicolor flow cytometry panels

Flow cytometric analyses with monoclonal antibodies have been restricted primarily to cell surface molecules. Intracellular structures such as cytoplasmic or nuclear enzymes, oncoproteins, cytokines, immunoglobulins etc. were largely excluded from such studies.

MACS™ Marker Screen has been designed for screening and analysis of 371 human markers by flow cytometry. The kit combines the benefits of flow cytometry and high quality pre-titrated antibodies to screen for markers of interest in a standardized and high throughput manner.