The EDUCATE environment provides links to a number of commercial and non-commercial resources relating to flow cytometry and antibody-based techniques. You can link directly to the suppliers' websites.
Suggestions for additional content are always welcome. Please email these to firstname.lastname@example.org
Multicolor flow cytometry panels
True-Stain Monocyte Blocker™ has been formulated to block non-specific binding of PE/Dazzle™ 594, PE/Cy5, PE/Cy7, APC/Cy7 and APC/Fire™ 750 commonly seen on monocytes and macrophages. The blocker is most effective when doing surface staining on live cells prior to the fixation step in a staining protocol. Learn more.
Beyond antibody reagents, flow cytometry requires the right types of buffers for optimal staining. This convenient list separates out flow cytometry applications by their intended target. It also indicates which buffers are best-suited to your task for surface or intracellular staining and the protocols necessary for each.
MACS™ Marker Screen has been designed for screening and analysis of 371 human markers by flow cytometry. The kit combines the benefits of flow cytometry and high quality pre-titrated antibodies to screen for markers of interest in a standardized and high throughput manner.
In addition to the broad range of flow cytometry validated antibodies we offer for immunology, cancer and veterinary science, we also have supporting reagents that will optimize staining and sample preparation.
Miltenyi Biotec offers a huge range of fully validated antibodies suitable for multiple applications, such as flow cytometry, microscopy, and functional assays. The MACS™ Antibody Portfolio guarantees high performance consistency, with the majority manufactured using our innovative and proprietary recombinant antibody technology for unrivalled lot-to-lot reproducibility and standardization.
Clinical flow cytometry
Diagnostic and quality-control reagents for cell manufacturing
Mouse Seroblock FcR is a rat antibody that recognises mouse CD16 and CD32, cell surface proteins also known as FcRγIII and FcR&gamma:II, respectively. The function of these proteins is to bind IgG molecules via their Fc regions as part of the adaptive immune response.
Flow cytometric analyses with monoclonal antibodies have been restricted primarily to cell surface molecules. Intracellular structures such as cytoplasmic or nuclear enzymes, oncoproteins, cytokines, immunoglobulins etc. were largely excluded from such studies.
Human Fc receptors are expressed on a variety of immune cell types including monocytes, macrophages, B cells, granulocytes and dendritic cells. Cells that express Fc receptors can give false positive immunofluorescent staining due to the Fc receptors binding of Ig. Human SeroBlock is designed to prevent such non-specific staining without interfering with appropriate target staining. Human SeroBlock is compatible with use of anti-human antibodies targeting Fc receptors in flow cytometry.