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Flow cytometric analyses with monoclonal antibodies have been restricted primarily to cell surface molecules. Intracellular structures such as cytoplasmic or nuclear enzymes, oncoproteins, cytokines, immunoglobulins etc. were largely excluded from such studies.

Optimization of intracellular flow cytometry staining results with Leucoperm

Flow cytometry protocols and staining procedures vary depending on whether the antigen to be detected is located on the cell surface or intracellular.