The EDUCATE environment provides links to a number of commercial and non-commercial resources relating to flow cytometry and antibody-based techniques. You can link directly to the suppliers' websites.
Suggestions for additional content are always welcome. Please email these to email@example.com
Flow cytometry protocols and staining procedures vary depending on whether the antigen to be detected is located on the cell surface or intracellular.
Flow cytometric analyses with monoclonal antibodies have been restricted primarily to cell surface molecules. Intracellular structures such as cytoplasmic or nuclear enzymes, oncoproteins, cytokines, immunoglobulins etc. were largely excluded from such studies.