Direct and indirect staining, staining of intracellular antigens, permeabilization and cell preparation protocols
The flow cytometry protocols below provide detailed procedures for the treatment and staining of cells prior to using a flow cytometer.
Protocols are available for:
- Direct staining of cells applicable where the fluorophore is directly linked to the primary antibody
- Indirect staining of cells applicable when using unconjugated or biotin-conjugated monoclonal and polyclonal antibodies
- Intracellular staining methods for intracellular antigens and cytokines
- DNA staining for cell cycle analysis
- Cell preparation