Optimized Multicolor Immunofluorescence Panel

Optimized Multicolor Immunofluorescence Panel (OMIP) is a special peer-reviewed Cytometry Part A publication type that reports on newly designed and optimized multicolor panels for flow cytometry, fluorescence microscopy, image cytometry, and other polychromatic fluorescence-based methods. The first two OMIPs were published in the September 2010 issue of the Journal.

An OMIP consists of two portions:

  1. A two-page printed component includes basic information, such as the title, a summary table, a brief narrative, a reagent table, an example staining figure and a detailed comparison with similar OMIPs.
  2. An online component includes significant amount of technical details, such as panel development strategy, cross-references to related panels, exact staining protocol, instrument configuration and reagent information.

For more information on OMIPs, please refer to the following articles:

OMIPs—Orchestrating multiplexity in polychromatic science
Mario Roederer and Attila Tárnok
Cytometry, 77A: 811–812.

Publication of optimized multicolor immunofluorescence panels
Yolanda Mahnke, Pratip Chattopadhyay, and Mario Roederer
Cytometry, 77A: 814–818

OMIPs published to date:

OMIP-001: Quality and phenotype of Ag-responsive human T-cells.
Yolanda Mahnke and Mario Roederer
Cytometry, 77A: 819–820

OMIP-002: Phenotypic analysis of specific human CD8 T-cells using peptide-MHC class I multimers for any of four epitopes.
Pratip Chattopadhyay, Mario Roederer, and David Price
Cytometry, 77A: 821–822

OMIP-003: Phenotypic analysis of human memory B cells
Chungwen Wei, John Jung, and Iñaki Sanz
Cytometry, 79A: 894–896

OMIP-004: In-depth characterization of human T regulatory cells
Angélique Biancotto, Pradeep Dagur, J. Chris Fuchs, Marc Langweiler, and J. Philip McCoy Jr,
Cytometry, 81A: 15–16

OMIP-005: Quality and phenotype of antigen-responsive rhesus macaque T cells
Kathryn Foulds, Mitzi Donaldson, and Mario Roederer
Cytometry, 81A: 360–361

OMIP-006: Phenotypic subset analysis of human T regulatory cells via polychromatic flow cytometry
David Murdoch, Janet Staats, and Kent Weinhold
Cytometry, 81A: 281–283.

OMIP-007: Phenotypic analysis of human natural killer cells
Michael Eller and Jeffrey Currier
Cytometry, 81A: 447–449

OMIP-008: Measurement of Th1 and Th2 cytokine polyfunctionality of human T cells
Cindy Zuleger and Mark Albertini
Cytometry, 81A: 450–452

OMIP-009: Characterization of antigen-specific human T-cells
Laurie Lamoreaux, Richard Koup, and Mario Roederer
Cytometry, 81A: 362–363

OMIP-010: A new 10-color monoclonal antibody panel for polychromatic immunophenotyping of small hematopoietic cell samples.
Frank Preijers, Erik Huys, and Bijan Moshaver
Cytometry, 81A: 453–455

OMIP-011: Characterization of circulating endothelial cells (CECs) in peripheral blood
Raskit Lachmann, Paola Lanuti, and Sebastiano Miscia
Cytometry, 81A: 549–551

OMIP-012: Phenotypic and numeric determination of human leukocyte reconstitution in humanized mice
Brian Long and Cheryl Stoddart
Cytometry, 81A: 646–648

OMIP-013: Differentiation of human T-cells.
Yolanda Mahnke, Margaret Beddall, and Mario Roederer
Cytometry, 81A: 935–936

OMIP-014: Validated multifunctional characterization of antigen-specific human T cells by intracellular cytokine staining
Stephen De Rosa, Donald Carter, and M. Juliana McElrath
Cytometry, 81A: 1019–1021

OMIP-015: Human regulatory and activated T-cells without intracellular staining
Yolanda Mahnke, Margaret Beddall, and Mario Roederer
Cytometry, 83A: 179–181

OMIP-016: Characterization of antigen-responsive macaque and human T-cells
Sabrina Guenounou, Nathalie Bosquet, Claudia Dembek, Roger Le Grand, and Antonio Cosma
Cytometry, 83A: 182–184

OMIP-017: Human CD4+ helper T-cell subsets including follicular helper cells
Yolanda Mahnke, Margaret Beddall, and Mario Roederer
Cytometry, 83A: 439–440

OMIP-018: Chemokine receptor expression on human T helper cells

Tess Brodie, Elena Brenna, and Federica Sallusto
Cytometry, 83A: 530–532

Corrigendum for OMIP-018: Chemokine receptor expression on human T helper cells
Tess Brodie, Elena Brenna, and Federica Sallusto
This article corrects
OMIP-018: Chemokine receptor expression on human T helper cells
Cytometry, 83A: 530–532

OMIP-019: Quantification of human γδT-cells, iNKT-cells, and hematopoietic precursors
Yolanda Mahnke, Margaret Beddall, and Mario Roederer
Cytometry, 83A: 676–678

OMIP-020: Phenotypic characterization of human γδ T-cells by multicolor flow cytometry.
Kilian Wistuba-Hamprecht, Graham Pawelec, and Evelyna Derhovanessian
Cytometry, 85: 522–524

OMIP-021: Simultaneous quantification of human conventional and innate-like T-cell subsets
Nicholas Gherardin, David Ritchie, Dale Godfrey, and Paul Neeson
Cytometry, 85: 573-575

OMIP-022: comprehensive assessment of antigen-specific human t-cell functionality and memory
Andrew Graves, Marcelino Padilla, and David Hokey
Cytometry, 85: 576-579

OMIP-023: 10-color, 13 antibody panel for in-depth phenotyping of human peripheral blood leukocytes
József Bocsi, Susanne Melzer, Ingo Dähnert and Attila Tárnok
Cytometry, 85: 781-784

OMIP-024: Pan-leukocyte immunophenotypic characterization of PBMC subsets in human samples
Gemma Moncunill, Hannah Han, Carlota Dobaño, M. Juliana McElrath and Stephen C. De Rosa
Cytometry, 85: 995-998

OMIP-025: Evaluation of human T- and NK-cell responses including memory and follicular helper phenotype by intracellular cytokine staining
Gemma Moncunill, Carlota Dobaño, M. Juliana McElrath and Stephen C. De Rosa
Cytometry, 87: 289-292

OMIP-026: Phenotypic analysis of B and plasma cells in rhesus macaques
Berit Neumann, Sieghart Sopper and Christiane Stahl-Hennig
Cytometry, 87: 800-802

OMIP-027: Functional analysis of human natural killer cells
Margaret C. Costanzo, Matthew Creegan, Kerri G. Lal and Michael A. Eller
Cytometry, 87: 803-805

OMIP-028: Activation panel for Rhesus macaque NK cell subsets
Nicholas Pomplun, Kim L. Weisgrau, David T. Evans and Eva G. Rakasz
Cytometry, 87: 890–893

OMIP-029: Human NK-cell phenotypization
Yolanda D. Mahnke, Margaret H. Beddall and Mario Roederer
Cytometry, 87: 890–893

OMIP-030: Characterization of human T cell subsets via surface markers
Gerhard Wingender and Mitchell Kronenberg
Cytometry, 87: 1067–1069

OMIP-031: Immunologic checkpoint expression on murine effector and memory T-cell subsets
Satoshi Nemoto, Adam W. Mailloux, Jodi Kroeger and James J. Mulé
Cytometry, 89: 427–429

OMIP-032: Two multi-color immunophenotyping panels for assessing the innate and adaptive immune cells in the mouse mammary gland
Ashleigh Unsworth, Robin Anderson, Nicole Haynes and Kara Britt
Cytometry, 89: 527–530

OMIP-033: A comprehensive single step staining protocol for human T- and B-cell subsets
Tess Brodie, Kristina Rothaeusler and Mireia Sospedra
Cytometry, 89: 629-632

OMIP-034: Comprehensive immune phenotyping of human peripheral leukocytes by mass cytometry for monitoring immunomodulatory therapies
Sabine Baumgart, Anette Peddinghaus, Ursula Schulte-Wrede, Henrik E. Mei and Andreas Grützkau

OMIP-035: Functional analysis of natural killer cell subsets in macaques
Kim L. Weisgrau, Moritz Ries, Nicholas Pomplun, David T. Evans and Eva G. Rakasz
Cytometry, 89: 799-802

OMIP-036: Co-inhibitory receptor (immune checkpoint) expression analysis in human T cell subsets
Zachary R. Healy and David M. Murdoch
Cytometry, 89: 889-892

OMIP-037: 16-color panel to measure inhibitory receptor signatures from multiple human immune cell subsets
Anna C. Belkina and Jennifer E. Snyder-Cappione

OMIP-038: Innate immune assessment with a 14 color flow cytometry panel
Kinga K. Smolen, Bing Cai and Tobias R. Kollmann

OMIP-039: Detection and analysis of human adaptive NKG2C+ natural killer cells
Quirin Hammer and Chiara Romagnani

OMIP-040: Optimized gating of human prostate cellular subpopulations
Gervaise H. Henry, Nicolas Loof and Douglas W. Strand

OMIP-041: Optimized multicolor immunofluorescence panel rat microglial staining protocol
Naama E. Toledano Furman, Karthik S. Prabhakara, Supinder Bedi, Charles S. Cox Jr and Scott D. Olsoni

OMIP-042: 21-color flow cytometry to comprehensively immunophenotype major lymphocyte and myeloid subsets in human peripheral blood
Karl W. Staser, William Eades, Jaebok Choi, Darja Karpova and John DiPersio

OMIP-043: Identification of human antibody secreting cell subsets
Jeffrey Carrell and Christopher J. Groves

OMIP-044: 28-color immunophenotyping of the human dendritic cell compartment
Florian Mair and Martin Prlic