Miltenyi Biotec Detecting Cytokine-Secreting Cells

Cytokine detection assays

Flow cytometric detection of cytokine-secreting cells

  • Highly sensitive detection of viable cytokine-secreting cells
  • Analysis of cytokine secretion at single-cell level
  • Rapid and reliable quantitation of intracellular cytokine levels

MACS™ Cytokine Secretion Assays are designed for the sensitive detection of viable cytokine-secreting cells. This unique technology allows for the detection of secreted cytokines at a single-cell level, and simultaneous phenotyping of cytokine-secreting cells. 

Our intracellular cytokine detection kits are designed for rapid and reliable flow cytometric detection of cytokine production in activated CD4+ and CD8+ T cells. The kits contain all reagents necessary for the detection of cytokine-producing activated T cells from whole blood or PBMCs by intracellular staining, including reagents for the fixation and permeabilization of cells prior to immunofluorescent staining, as well as for the in vitro stimulation of T cells as a positive control.