Quality Controls and Accreditation in a Clinical FCM Laboratory
According to the ISO Norm 15189, experience from Germany
Professor Attila Tárnok
Fraunhofer Institute for Cell Therapy and Immunology IZI, Leipzig, Germany, and Editor-in-Chief of Cytometry Part A.
Objectives of the Lecture
This webinar will mention the basics of flow cytometry and multi-colour panels and provide essential aspects of QM according to the ISO 15189 norm.
Abstract of Lecture
Flow cytometry has proven to be an indispensable tool in both clinical diagnosis and biomedical research, as it enables for objective and quantitative analysis of cells in heterogeneous cell populations. Areas of application in clinical diagnosis range from immunophenotyping over immune surveillance to Hematology and Oncology. Although today’s standard instruments are manufactured to analyse up to millions of cells within minutes and with the utmost precision, laboratory testing of patient samples can remain a complex procedure and should be closely monitored. Laboratory Quality management (QM) is paramount to prevent many issues, notably through the use of Quality Controls (QC) which will ensure both precision and accuracy of patient sample results.
In these sessions, we will explore cells sorting by flow cytometry. This is an extremely useful technique as it allows us to separate cells of interest for further study. However, the success of a sort depends on many factors including the sample preparation and knowledge of how the sorter works. On 12 February we will look at the theory of cells sorting and perform a simple sorting experiment using a Sony SH800 cell sorter; in the session on 12 March we will consolidate that knowledge and use a BD FACSAria to perform a more complex cell sort. The aim of both sessions is to show attendees the theory of cell sorting but also the practicalities of performing a successful sort. These webinars are a collaboration between Derek Davies (The Francis Crick Institute, London, UK), and Kathy Daniels and Rui Gardner (Memorial Sloan Kettering Cancer Center, New York, USA).
Please email email@example.com for questions about this course.
As we face another year of socially distanced online meetings, the Canadian Cytometry and Microscopy Association (CCMA) has decided to postpone our biennial meeting for another year in favor of a new initiative that we hope will keep Canadian cytometry communities socially connected until we can meet again in person. We will be hosting a series of informal 1-hr chats called “Cytometry SRL Perspectives”. These will be conversations between guest panelists who are key opinion leaders representing different perspectives on a range of topics in flow cytometry and microscopy spanning emerging technologies & applications, instrument acquisition, to core facility management, education, and much more. Sessions will consist of panelists giving their perspective in a given subject area followed by topic-driven discussions mediated by the moderators. This will be followed by ~10 minutes of questions and comments from our attendees at the end.
In an effort to reach out to core facilities and cytometrists across Canada, we will also include in each session a 5-min segment of “Meet the Cores of Canada” where we will feature core facilities and their members from across the country.
These events will require registration but are free for all to attend - please feel free to share this invitation.
When: Noon (Eastern Time) – First session will be Feb 17, 2021 with sessions occurring every 6 weeks. To receive registration info and/or details on upcoming sessions:https://forms.gle/bAiPmoJTLWD7GXst7
Upcoming: Resolving the Small Stuff - Small Particle Flow Cytometry - Wed. Feb 17, 2021 @noon ET There is increasing interest in the use of flow cytometry for phenotypic analysis of small particles such as extracellular vesicles and viruses. To detect these small particles, conventional flow cytometers are functioning at the limit of detection. There is currently no consensus on how small particles should be analysed on flow cytometers. This session brings together small particle flow cytometry gurus from around the world to share their different approaches to resolving the small stuff.
Topics for discussion:
Pros and cons of SSC vs FL threshold
Calibration methods and the materials to use (FL & SSC)
Edwin van der Pol (Amsterdam University Medical Centers/Exometry)
Desmond Pink (Nanostics)
Joanne Lannigan (Flow Cytometry Support Services)
John Nolan (Scintillon Institute/Cellarcus)
Joshua Welsh (NIH)
Vera Tang (University of Ottawa)
We look forward to connecting with you all and building our Canadian network of cytometrists of all flavors- flow, microscopy, and genomic!
Best, CCMA Executive Board Aja Reiger (Co-President) Vera Tang (Co-President) Desmond Pink (Vice President) Marie-Hélène Lacombe (Treasurer) Catarina Di Ciano-Oliveira (Secretary) Emily Reddy (Communications Director)
We have converted our face to face Module 1:Fundamentals of Flow Cytometry course to a zoom based interactive one day course which is fully booked for the two dates in December. I have therefore arranged two further two Virtual Interactive Fundamentals of Flow Cytometry Courses to be held on 14th January and 25th February 2021. The cost is £50 (plus VAT and Eventbrite fee) per person. To register for these courses (there are limited places on these courses due to the interactive nature of the courses), please visit the Eventbrite links below. Please pass this email on to anyone who you think might be interested in attending.