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German BioImaging is hosting again their Core Facility Leadership and Management Course, designed specifically for core facility/SRL managers in the life sciences. This year, the course will be held in an online format between September 29 and October 15 in eight complementary half-day sessions (9:00-12:30 CEST).
OpenFlow Cytometry: An Introduction to Flow Cytometric Panel Design
We have shown you how to set up a flow cytometer, looked at compensation and data analysis now is the time to look at Panel Design! In this session we will look at what makes a good fluorochrome choice, how we need to consider sample preparation and fixation and how we can begin to think about making an optimised combination for your cytometer. With a mixture of theory and practice, this session is suitable for those beginning in flow cytometry and is a place to hear about current best practices. Time
General review on applications of immunology analysis by flow cytometry, B-cells lymphopoiesis, development and pathology; T cell phenotypes and subsets; Assays for the assessment of T cell activation, proliferation, and function (Antigen specific response); Innate Lymphoid Cells; Myeloid lineage, myelopoiesis and normal maturation pathways; Phagocytosis, monitoring granulocyte reactivity and ROS production; Flow cytometric analysis of human monocytes and dendritic cells; Immunopathology and Sepsis, Immunoparalysis; Hypersensitivity and Basophil degranulation tests; Primary immunodeficiencies; Multicolor Panel design; QC and standardisation; ImageStream; Mass cytometry; High-dimensional data analysis
Thomas MATTHES (Geneva) Claude LAMBERT (Saint-Etienne)