Events

September 2021

Monday
Tuesday
Wednesday
Thursday
Friday
Saturday
Sunday
30 Monday
Computing & Cytometry 2021 HERE

Introduction to modern computational cytometry techniques

The aim of this workshop is to introduce the participants to modern computational cytometry techniques. A mixture of theoretical and practical courses will show the participants how to set up a pipeline using the R programming language, including key steps such as data preprocessing and transformation, automated identification of cell populations with the FlowSOM algorithm and differential analysis of sample groups.

No previous knowledge of R is assumed, but participants already familiar with R can join the workshop at 14h.

Program

Day 1

  • 9h00 - 9h15 Welcome and Introduction by Thomas Matthes (UNIGE; Geneva, CH)
  • 9h15 - 12h15 “Introduction to programming language R” by Helena Todorov (UNIL; Lausanne, CH)
  • 12h15 – 14h00 Lunch
  • 14h00 - 15h00 “The mathematics of multidimensional data analysis” by Stephane Marchand-Maillet (UNIGE; Geneva, CH)
  • 15h00 - 16h00 “Pre-processing of data files” by H Todorov (UNIL; Lausanne, CH)
    16h00 – 16h15 Coffee break
  • 16h5 – 18h5 “Design a flow-cytometry study in view of non-supervised analysis: How to start” by Sarah Cattin (Fribourg, CH)
  • 19h00 Dinner

Day 2

  • 8h15 - 10h00 “Practical training on datasets in R: from pre-processing to visualization” by Guillaume Beyrend (VisuaLyte)
  • 10h00 - 10h15 Coffee break
  • 10h15 - 11h45 “Applying FlowSom and t-sne using R” by H Todorov (UNIL; Lausanne, CH)
  • 11h45 – 13h15 “The Bordeaux experience” by Francis Lacombe (Bordeaux, France)
  • 13h15 – 14h30 Lunch
  • 14h30 - 16h30 “Advanced analysis tools and plugins for discovery with FlowJo” by Serena Di Cecilia (BD Life Sciences)
  • 16h30 – 16h45 Coffee break
  • 16h45 - 17h45 “A data driven approach” by H Todorov and T Matthes
  • 17h45 – 18h00 General Discussion and Closing remarks by T Matthes
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31 Tuesday
Computing & Cytometry 2021 HERE

Introduction to modern computational cytometry techniques

The aim of this workshop is to introduce the participants to modern computational cytometry techniques. A mixture of theoretical and practical courses will show the participants how to set up a pipeline using the R programming language, including key steps such as data preprocessing and transformation, automated identification of cell populations with the FlowSOM algorithm and differential analysis of sample groups.

No previous knowledge of R is assumed, but participants already familiar with R can join the workshop at 14h.

Program

Day 1

  • 9h00 - 9h15 Welcome and Introduction by Thomas Matthes (UNIGE; Geneva, CH)
  • 9h15 - 12h15 “Introduction to programming language R” by Helena Todorov (UNIL; Lausanne, CH)
  • 12h15 – 14h00 Lunch
  • 14h00 - 15h00 “The mathematics of multidimensional data analysis” by Stephane Marchand-Maillet (UNIGE; Geneva, CH)
  • 15h00 - 16h00 “Pre-processing of data files” by H Todorov (UNIL; Lausanne, CH)
    16h00 – 16h15 Coffee break
  • 16h5 – 18h5 “Design a flow-cytometry study in view of non-supervised analysis: How to start” by Sarah Cattin (Fribourg, CH)
  • 19h00 Dinner

Day 2

  • 8h15 - 10h00 “Practical training on datasets in R: from pre-processing to visualization” by Guillaume Beyrend (VisuaLyte)
  • 10h00 - 10h15 Coffee break
  • 10h15 - 11h45 “Applying FlowSom and t-sne using R” by H Todorov (UNIL; Lausanne, CH)
  • 11h45 – 13h15 “The Bordeaux experience” by Francis Lacombe (Bordeaux, France)
  • 13h15 – 14h30 Lunch
  • 14h30 - 16h30 “Advanced analysis tools and plugins for discovery with FlowJo” by Serena Di Cecilia (BD Life Sciences)
  • 16h30 – 16h45 Coffee break
  • 16h45 - 17h45 “A data driven approach” by H Todorov and T Matthes
  • 17h45 – 18h00 General Discussion and Closing remarks by T Matthes
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1 Wednesday
2 Thursday
3 Friday
4 Saturday
5 Sunday
6 Monday
7 Tuesday
8 Wednesday
9 Thursday
10 Friday
11 Saturday
12 Sunday
17 Friday
18 Saturday
19 Sunday
20 Monday
21 Tuesday
Intensive Hands-On Flow Cytometry Course (York)
  • "An excellent course with fantastic tutors." "Thanks for all the team who were really special and what can I say -never better."
  • "I am very impressed with the course staff, interactions, coursematerial and the way it was planned."
  • "Excellent course, excellent teaching - thank you."
  • "A very well run, enjoyable and informative course.  I woulddefinitely recommend to future users.  I wish I had taken this coursea few years ago."
  • Introduction  An intensive, hands-on, four-day training course in the varied techniques using flow cytometry.  The course will initially cover basic flow cytometry but then rapidly move on to the more advanced applications. These will include cell cycle, apoptosis, cell proliferation, GFP and other fluorescent proteins, FRET and receptor quantification. The course has been designed to allow the participant to gain experience of what a flow cytometer is capable and to raise awareness of the problems that can be encountered. To demonstrate each technique, a wide range of both samples (plants to cell culture) and labels (antibody to fluorescent proteins) will be used. Participants are welcome to bring their own samples. 

    The course is taught by academic and research staff of the University of York Technology Facility, guest specialists from over the UK as well as Beckman Coulter's own applications specialist. Guest specialists will give an initial talk on their specialised techniques and will then participate in the running of the practical sessions.

    The University of York is pleased to present the course in collaboration with Beckman Coulter.

    Based on delegates from similar courses in 2018 and 2019, it is anticipated that participants will come from both academia and industry throughout the UK, Europe and the rest of the world.

    Course Benefits  This intensive, four-day training course will be of value to anyone wanting to gain hands-on experience of modern flow cytometric techniques. In addition to the hands-on component, the course will include tutorials on the theory and practice of all the techniques and methods covered, as well as many other relevant topics in the field.

    An essential feature of our course is the unique combination of practical, laboratory-based work with both taught and tutorial-style classroom sessions. The Technology Facility labs are extremely well equipped for modern flow cytometry, including the two CyAn ADP analysers, with 3 lasers (405, 488 and 635nm) and 9 colour detectors. Participants will have the opportunity to analyse their own samples during the free format session or to cover previous work where appropriate.

    " />
    22 Wednesday
    Intensive Hands-On Flow Cytometry Course (York)
  • "An excellent course with fantastic tutors." "Thanks for all the team who were really special and what can I say -never better."
  • "I am very impressed with the course staff, interactions, coursematerial and the way it was planned."
  • "Excellent course, excellent teaching - thank you."
  • "A very well run, enjoyable and informative course.  I woulddefinitely recommend to future users.  I wish I had taken this coursea few years ago."
  • Introduction  An intensive, hands-on, four-day training course in the varied techniques using flow cytometry.  The course will initially cover basic flow cytometry but then rapidly move on to the more advanced applications. These will include cell cycle, apoptosis, cell proliferation, GFP and other fluorescent proteins, FRET and receptor quantification. The course has been designed to allow the participant to gain experience of what a flow cytometer is capable and to raise awareness of the problems that can be encountered. To demonstrate each technique, a wide range of both samples (plants to cell culture) and labels (antibody to fluorescent proteins) will be used. Participants are welcome to bring their own samples. 

    The course is taught by academic and research staff of the University of York Technology Facility, guest specialists from over the UK as well as Beckman Coulter's own applications specialist. Guest specialists will give an initial talk on their specialised techniques and will then participate in the running of the practical sessions.

    The University of York is pleased to present the course in collaboration with Beckman Coulter.

    Based on delegates from similar courses in 2018 and 2019, it is anticipated that participants will come from both academia and industry throughout the UK, Europe and the rest of the world.

    Course Benefits  This intensive, four-day training course will be of value to anyone wanting to gain hands-on experience of modern flow cytometric techniques. In addition to the hands-on component, the course will include tutorials on the theory and practice of all the techniques and methods covered, as well as many other relevant topics in the field.

    An essential feature of our course is the unique combination of practical, laboratory-based work with both taught and tutorial-style classroom sessions. The Technology Facility labs are extremely well equipped for modern flow cytometry, including the two CyAn ADP analysers, with 3 lasers (405, 488 and 635nm) and 9 colour detectors. Participants will have the opportunity to analyse their own samples during the free format session or to cover previous work where appropriate.

    " />
    23 Thursday
    Intensive Hands-On Flow Cytometry Course (York)
  • "An excellent course with fantastic tutors." "Thanks for all the team who were really special and what can I say -never better."
  • "I am very impressed with the course staff, interactions, coursematerial and the way it was planned."
  • "Excellent course, excellent teaching - thank you."
  • "A very well run, enjoyable and informative course.  I woulddefinitely recommend to future users.  I wish I had taken this coursea few years ago."
  • Introduction  An intensive, hands-on, four-day training course in the varied techniques using flow cytometry.  The course will initially cover basic flow cytometry but then rapidly move on to the more advanced applications. These will include cell cycle, apoptosis, cell proliferation, GFP and other fluorescent proteins, FRET and receptor quantification. The course has been designed to allow the participant to gain experience of what a flow cytometer is capable and to raise awareness of the problems that can be encountered. To demonstrate each technique, a wide range of both samples (plants to cell culture) and labels (antibody to fluorescent proteins) will be used. Participants are welcome to bring their own samples. 

    The course is taught by academic and research staff of the University of York Technology Facility, guest specialists from over the UK as well as Beckman Coulter's own applications specialist. Guest specialists will give an initial talk on their specialised techniques and will then participate in the running of the practical sessions.

    The University of York is pleased to present the course in collaboration with Beckman Coulter.

    Based on delegates from similar courses in 2018 and 2019, it is anticipated that participants will come from both academia and industry throughout the UK, Europe and the rest of the world.

    Course Benefits  This intensive, four-day training course will be of value to anyone wanting to gain hands-on experience of modern flow cytometric techniques. In addition to the hands-on component, the course will include tutorials on the theory and practice of all the techniques and methods covered, as well as many other relevant topics in the field.

    An essential feature of our course is the unique combination of practical, laboratory-based work with both taught and tutorial-style classroom sessions. The Technology Facility labs are extremely well equipped for modern flow cytometry, including the two CyAn ADP analysers, with 3 lasers (405, 488 and 635nm) and 9 colour detectors. Participants will have the opportunity to analyse their own samples during the free format session or to cover previous work where appropriate.

    " />
    24 Friday
    Intensive Hands-On Flow Cytometry Course (York)
  • "An excellent course with fantastic tutors." "Thanks for all the team who were really special and what can I say -never better."
  • "I am very impressed with the course staff, interactions, coursematerial and the way it was planned."
  • "Excellent course, excellent teaching - thank you."
  • "A very well run, enjoyable and informative course.  I woulddefinitely recommend to future users.  I wish I had taken this coursea few years ago."
  • Introduction  An intensive, hands-on, four-day training course in the varied techniques using flow cytometry.  The course will initially cover basic flow cytometry but then rapidly move on to the more advanced applications. These will include cell cycle, apoptosis, cell proliferation, GFP and other fluorescent proteins, FRET and receptor quantification. The course has been designed to allow the participant to gain experience of what a flow cytometer is capable and to raise awareness of the problems that can be encountered. To demonstrate each technique, a wide range of both samples (plants to cell culture) and labels (antibody to fluorescent proteins) will be used. Participants are welcome to bring their own samples. 

    The course is taught by academic and research staff of the University of York Technology Facility, guest specialists from over the UK as well as Beckman Coulter's own applications specialist. Guest specialists will give an initial talk on their specialised techniques and will then participate in the running of the practical sessions.

    The University of York is pleased to present the course in collaboration with Beckman Coulter.

    Based on delegates from similar courses in 2018 and 2019, it is anticipated that participants will come from both academia and industry throughout the UK, Europe and the rest of the world.

    Course Benefits  This intensive, four-day training course will be of value to anyone wanting to gain hands-on experience of modern flow cytometric techniques. In addition to the hands-on component, the course will include tutorials on the theory and practice of all the techniques and methods covered, as well as many other relevant topics in the field.

    An essential feature of our course is the unique combination of practical, laboratory-based work with both taught and tutorial-style classroom sessions. The Technology Facility labs are extremely well equipped for modern flow cytometry, including the two CyAn ADP analysers, with 3 lasers (405, 488 and 635nm) and 9 colour detectors. Participants will have the opportunity to analyse their own samples during the free format session or to cover previous work where appropriate.

    " />
    25 Saturday
    26 Sunday
    27 Monday
    28 Tuesday
    29 Wednesday
    Core Facility Management course https://gerbi-gmb.de/CFLMC2021

    Best wishes,

    Christian
    _________________________________________________

    Christian Kukat, PhD
    Head of FACS & Imaging Core Facility
    Max Planck Institute for Biology of Ageing
    Joseph-Stelzmann-Str. 9b, D-50931 Köln / Cologne, Germany

    E-mail: Christian.Kukat@age.mpg.de
    www.age.mpg.de

    " />
    30 Thursday
    Core Facility Management course https://gerbi-gmb.de/CFLMC2021

    Best wishes,

    Christian
    _________________________________________________

    Christian Kukat, PhD
    Head of FACS & Imaging Core Facility
    Max Planck Institute for Biology of Ageing
    Joseph-Stelzmann-Str. 9b, D-50931 Köln / Cologne, Germany

    E-mail: Christian.Kukat@age.mpg.de
    www.age.mpg.de

    " />
    Covid, One Year On https://www.rms.org.uk/rms-event-calendar/2021-events/virtual-flow-cytometry-covid-update-one-year-on.html

    To help start and shape discussions we have set up a short survey www.surveymonkey.co.uk/r/FlowCovid - please complete this by Monday 27 September.

    " />
    1 Friday
    Core Facility Management course https://gerbi-gmb.de/CFLMC2021

    Best wishes,

    Christian
    _________________________________________________

    Christian Kukat, PhD
    Head of FACS & Imaging Core Facility
    Max Planck Institute for Biology of Ageing
    Joseph-Stelzmann-Str. 9b, D-50931 Köln / Cologne, Germany

    E-mail: Christian.Kukat@age.mpg.de
    www.age.mpg.de

    " />
    An Introduction to Flow Cytometric Panel Design London

    " />
    2 Saturday
    Core Facility Management course https://gerbi-gmb.de/CFLMC2021

    Best wishes,

    Christian
    _________________________________________________

    Christian Kukat, PhD
    Head of FACS & Imaging Core Facility
    Max Planck Institute for Biology of Ageing
    Joseph-Stelzmann-Str. 9b, D-50931 Köln / Cologne, Germany

    E-mail: Christian.Kukat@age.mpg.de
    www.age.mpg.de

    " />
    3 Sunday
    Core Facility Management course https://gerbi-gmb.de/CFLMC2021

    Best wishes,

    Christian
    _________________________________________________

    Christian Kukat, PhD
    Head of FACS & Imaging Core Facility
    Max Planck Institute for Biology of Ageing
    Joseph-Stelzmann-Str. 9b, D-50931 Köln / Cologne, Germany

    E-mail: Christian.Kukat@age.mpg.de
    www.age.mpg.de

    " />