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Human Lineage Cocktail:Alexa Fluor® 700 is a cocktail of antibodies recognising all leucocytes in human peripheral blood, with the exception of dendritic cells and basophils.
Dendritic cells in peripheral blood are characterised by the phenotype HLA-DR+ve, Lineage Cocktail -ve. Basophils are HLA-DR -ve. Human Lineage Cocktail:Alexa Fluor® 700 may therefore be used in combination with HLA-DR specific antibodies (e.g. MCA1879PE ) to identify peripheral blood dendritic cells.
The individual antibodies in this cocktail are specific for cell subsets as follows:-
