Human Lineage Cocktail:Alexa Fluor® 700 is a cocktail of antibodies recognising all leucocytes in human peripheral blood, with the exception of dendritic cells and basophils.
Dendritic cells in peripheral blood are characterised by the phenotype HLA-DR+ve, Lineage Cocktail -ve. Basophils are HLA-DR -ve. Human Lineage Cocktail:Alexa Fluor® 700 may therefore be used in combination with HLA-DR specific antibodies (e.g. MCA1879PE ) to identify peripheral blood dendritic cells.
The individual antibodies in this cocktail are specific for cell subsets as follows:-
- CD3 T lymphocytes
- CD14 Monocytes
- CD16 NK cells, granulocytes
- CD19 B lymphocytes
- CD20 B lymphocytes
- CD56 NK cells