Educational Program
Plans | Includes | Access |
Access+ | . Flow Cytometry Essentials . Cytometry Select (with future releases) . FlowHub (with quarterly updates) . FlowEval (with future updates) | Register |
For additional information on pricing, payment, and bulk discounts, check out our FAQ (“What is the cost of a license and how can I pay?”).
If you are wondering why you should choose our program, consult our FAQ (“Why use WORK-FLOW’s Educational Program?”).
Flow Cytometry EssentialsTM is an on-demand, streamlined, brand-neutral training program, delivered as subtitled video-based micro-content and simulation.
Course Details
Modern and cost-effective, Flow Cytometry EssentialsTM (FCE) eliminates the tedious and time-consuming theoretical classes usually employed by core facilities and guarantees that all users receive the same quality information on common concepts and practical considerations before attending an instrument-specific session.
Approved users can create their own account and immediately start taking the course anywhere and at their own pace.
Beginners can stop and rewind the videos to fully understand the concepts or revisit the illustrations. Advanced users needing a refresher or required to take this course will make efficient use of their time thanks to the optimized delivery.
A total of 75 quiz questions, evenly allotted at the end of each section, help ascertain knowledge validation prior to the obtention of a certificate of completion.
An extensive glossary and access to the FlowHub, our resources platform, are available to complement the learning process.
1 – Principles & Instrumentation
- What is Flow Cytometry?
- Common Applications
- Fluorescence – Light Spectrum
- Fluorescence – Absorption & Emission
- Fluorescence – Fluorochrome Classes
- Fluorescence – Fluorochrome Properties
- Fluidics – Flow
- Fluidics – Pressure & Flow Rate
- Fluidics – Acoustic-Assisted Focusing
- Optics – Excitation
- Optics – Light Collection
- Optics – Photodetection
- Electronics – Amplification & Conversion
- Electronics – Voltage Pulse
- Electronics – Time Delay
- Electronics – Digitization & Binning
- Electronics – FCS File
- Section Summary
2 – Reagents & Panel Design
- Sample Requirements
- Cellular Markers and Dyes
- Fluorescence Detection
- Fluorochrome Selection
- Antigen Classification
- Antibody Titration
- Dead Cell Staining
- Exclusion / Dump Channel
- Spectral Overlap
- Compensation Controls
- Compensation
- Spectral Overlap Reduction
- Spillover Spreading
- Spillover Spreading Matrix
- Autofluorescence
- Fc-Mediated Binding
- Fluorescence Minus One Controls
- Biological Controls
- Replicates
- Section Summary
3 – Acquisition & Measurements
- Plots & Gates
- Scaling
- Acquisition Speed
- Event Cycling
- Scatter Setup
- Threshold & Trigger
- Aggregate Discrimination
- Dead Cell Exclusion
- Fluorescent Parameter Setup
- Compensation
- Data Recording
- Data Exporting
- Assay Setup Validation
- Instrument Cleaning
- Section Summary
4 – Analysis (with Interactive Tools)
- Matrix Inspection
- Data Display – Histograms & Plots
- Data Display – Gates, Regions, Quadrants
- Data Display – Gating
- Biexponential Scaling
- Data Cleaning – Time Gating
- Data Cleaning – Debris Exclusion
- Data Cleaning – Aggregates & Dead Cells
- Data Cleaning – Autofluorescence
- Data Cleaning – Antibody Aggregates
- Data Cleaning – Impact
- Gate Validation – FMO Controls
- Gate Validation – Backgating/Color Gating
- Cell Cycle – One-color DNA Analysis
- Cell Cycle – Multi-color DNA Analysis
- Section Summary
Interactive Tools
- Matrices
- Plotting
- Gating
- Scaling
- Cleaning Impact
- Controls
- DNA Analysis
5 – Statistics
- Number of Events to Record
- Descriptive Statistics – Distribution
- Descriptive Statistics – Central Tendency
- Descriptive Statistics – Dispersion
- Descriptive Statistics – Data Normalization
- Descriptive Statistics – Fold Change
- Inferential Statistics – Statistical Test
- Inferential Statistics – Statistical Power
- Inferential Statistics – Hypotheses
- Inferential Statistics – Significance Level
- Inferential Statistics – Statistical Errors
- Inferential Statistics – Probability Value
- Student’s t-test – T-distribution & Freedom
- Student’s t-test – Formula
- Student’s t-test – Real-Life Example pt1
- Student’s t-test – Real-Life Example pt2
- Section Summary
Preview
Access Instructions
- Already registered? Click LOGIN
- Group leader? Access the User Management Dashboard (instructions)
- Not yet registered? Click REGISTER in the table above or on the top menu (instructions)
Cytometry SelectTM is a series of cytometry courses on advanced, specialty topics.
CyS1 – Cell Sorting
Course Outline
- What is Cell Sorting? | Process Overview
- Nozzle Size & Sheath Pressure | Droplet Generation
- Droplet Breakoff
- Stream Charging & Deflection
- Collection
- Instrument Optimization | Drop Delay
- Acquisition & Gating
- Conflicts
- Sort Decisions
- Sorting
- Post-Sort – Purity, Recovery & Yield
- Process Stressors | Sample Quality
- Temperature & Pressure | Sample Media & Sheath
- Collection Media | Instrument Variables
- Sort Performance | Sort Quality
- Collection Issues | Contamination
- Single-Cell Deposition
- Biosafety | Summary
- Quiz (20 questions)
CyS2 – Full-Spectrum Flow Cytometry + Panel Optimization
Course Outline
- What is Full-Spectrum Flow Cytometry?
- Full-Spectrum vs. Conventional
- Spectral Emission Signature
- Signature Comparison
- Signature Alterations
- Reference Controls
- Spectral Reference Library
- Instrument Settings
- Acquisition
- Spectral Profile & Unmixing
- Autofluorescence Management
- Unmixing Errors
- Analysis
- Panel Optimization – Introduction
- Panel Optimization – Design
- Panel Optimization – Testing
- Panel Optimization – Evaluation & Remediation
- Summary
- Quiz (20 questions)
Access Instructions
- Already registered? Click LOGIN
- Group leader? Access the User Management Dashboard (instructions)
- Not yet registered? Click REGISTER in the table above or on the top menu (instructions)
FlowHubTM is a proprietary resources platform that centralizes information to efficiently deliver knowledge, leading users from experimental design to publication.
Contents
This cytometrist’s companion guide is a combination of original material, hosted information, and external references, totaling more than 730 documents and links. A convenient tool perfect for continuing education, time-saving guidance, or exploration into new applications.
Preview
Access
- Already registered? Click LOGIN
- Not yet registered? Click REGISTER in the table above or on the top menu (instructions)
FlowEvalTM is a proprietary flow cytometry knowledge assessment platform.
Contents
FlowEval is a comprehensive evaluation and preparation resource designed to help test your knowledge whether you are studying for an exam or are simply interested in strengthening your understanding.
Key Features:
• Extensive Question Bank: Access a wide range of flow cytometry questions
• Detailed Explanations: Understand the “why” behind the answers of more complex questions
• Realistic Practice Exams: Simulate exam conditions with full-length practice tests
• Access Anywhere: Study on-the-go with our responsive and user-friendly platform
Who Can Benefit?
• Students preparing for flow cytometry coursework and exams (i.e., ESCCA European Certificate for Cytometry Operators exam and Specialist in Cytometry, SCYM(ASCP) exam)
• Anyone seeking to enhance and validate their flow cytometry knowledge
Preview
Access
- Already registered? Click LOGIN
- Not yet registered? Click REGISTER in the table above or on the top menu (instructions)