The Vybrant® Phagocytosis Assay Kit provides a way for researchers to observe and quantitate the process of phagocytosis in human polynuclear cells and mouse macrophages by following the internalization of a foreign particle—in this case killed E. coli (K-12 strain) cells that have been labeled with the fluorescent dye fluorescein.
In addition to the lyophilized E. coli BioParticles™ component, the kit contains a trypan blue solution (to quench the fluorescence from particles that were not internalized) as well as step-by-step instructions for performing this phagocytosis assay in a fluorescence microplate reader. The methodology used with this kit has been developed using an adherent murine macrophage cell line (J774); however, by modifying the cell culture conditions, researchers can adapt this phagocytosis assay to other adherent cell lines.
Vybrant™ Phagocytosis Assay Kit Specifications:
- Particles are fluorescein-labeled E. coli (K-12 strain)
- Kit supplies sufficient reagents for ~250 tests using 96-well plates
- Fluorescence is measured using a microplate reader (Ex/Em ~480/520 nm)