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Secondary antibodies recognize an antibody as their target antigen, usually referred to as the primary antibody which in turn is bound to its antigen or protein of interest.
Unlike anti-idiotypic antibodies which bind specifically to the antigen-binding domain, secondary antibodies are specific to the conserved regions of an antibody which are constant within a particular class of antibody such as human IgG or mouse IgM.
Secondary antibodies are therefore able to bind to ranges of antibody clones making them very effective research tools.
