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5 Easy Steps for Successful Flow Cytometry

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Flow Cytometry is a powerful tool for analyzing the physical and chemical properties of cells and other components such as nuclei and chromosomes.

However, if you are a Flow Cytometry beginner, the whole process can be quite confusing. In order to get the best results, a lot of forethought and planning is required. In this blog post we simplify the process in five easy steps that will ensure successful results.

Step 1:

Plan your experiment in advance


As is the case with most experiments, careful planning beforehand goes a long way in generating reproducible results. For successful Flow Cytometry however, this is even more important. To guide you in your planning, here are a few questions you should carefully consider before beginning your experiment:

Are there reagents available for your target proteins or cells of interest?

We recommend that you first do a literature search or use the antibody search engine Citeab, which collates the most commonly cited antibodies for your specific targets. On Citeab you can also search by application, to ensure that you use the right clone for a particular application.

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