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SPHERO™ Particles for Calibration (Linearity & Sensitivity)

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Spherotech was established in 1992 to manufacture and supply uniform microparticles for biomedical and diagnostic applications. Our headquarters are 25 miles north of Chicago in Lake Forest, IL.

The SPHERO™ Calibration Particles are designed for routine calibration of flow cytometers. By using these particles, the sensitivity and linearity of the system can be determined.

The sensitivity of the instrument will help detect any dye or light contamination. The linearity of the instrument will determine if the logarithmic amps and PMTs are functioning properly.

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SPHERO™ Rainbow Calibration Particles

- Contains a mixture of fluorophores that enable the Rainbow Calibration Particles to be excited at any wavelength from 365 nm to 650 nm
- Allows the calibration of the FITC, PE, PE-TR, PE-Cy5, and APC channels in the flow cytometer with the same set of particles
- Consists of set of several similar size particles with different fluorescence intensities in a convenient dropper bottle
- Can be stored in the freezer and reused later since they can withstand repeated freezing and thawing

Catalog No.
Description
Concentration
Nominal Size
Package Size
Price
RCP-20-5
Rainbow Calibration Particles, 4 peaks
107/mL
1.8-2.2 µm
5 mL
RCP-30-5 (6 peaks)
Rainbow Calibration Particles, 6 peaks
107/mL
3.0-3.4 µm
5 mL
RCP-30-5A (8 peaks)
Rainbow Calibration Particles, 8 peaks
107/mL
3.0-3.4 µm
5 mL
RCP-32-5
Rainbow Calibration Particles, 6 peaks
107/mL
3.2 (+/- 0.1) µm
5 mL
RCP-35-5
Rainbow Calibration Particles, 4 peaks
107/mL
3.5-4.0 µm
5 mL
RCP-60-5
Rainbow Calibration Particles, 6 peaks
107/mL
6.0-6.4 µm
5 mL
RCP-30-5A-2
Rainbow Calibration Particles, 2nd peak from the 8 peak set
107/mL
3.0-3.4 µm
5 mL
Downloadable NOTE Rainbow Calibration Particles
ADDITIONAL NOTES:
1. Catalog No. RCP-30-5A Lot No. EAB01 has been reserved for the Euroflow Group. Please indicate this lot number when placing an order.
2. Other Rainbow Calibration Particles individual peaks are available upon request. Please contact customer service.
SPHERO™ Ultra Rainbow Calibration Particles
- Designed for routine calibration of the flow cytometer in all channels
- Contain a mixture of fluorophores that enable the optimization of linearity, sensitivity, and resolution of the flow cytometer
- Especially useful in the Far Red and Violet channels
Catalog No.
Description
Concentration
Nominal Size
Package Size
Price
URCP-38-2K
Ultra Rainbow Calibration Kit, 6 peaks
(Blank, 2x106/mL), (Fluorescent Particles, 107/mL)
107/mL after mixing
3.5-3.9 µm
1x3 mL
1x2 mL
URCP-50-2K
Ultra Rainbow Calibration Kit, 6 peaks
(Blank 2x106/mL), (Fluorescent Particles 107/mL)
107/mL after mixing
5.0-5.9 µm
1x3 mL
1x2 mL
SPHERO™ Spectral Matching Calibration Particle Kits
- Surface labeled polystyrene particles with commonly used fluorophores
- Used to determine the linearity of the logarithmic amps and sensitivity of flow cytometers in specific channels
- Supplied as individual products for use in specific channels of flow cytometers
Catalog No.
Description
Concentration
Nominal Size
Package Size
Price
ECFP-F1-5K
FITC Easy Calibration Kit, 5 peaks
2x106/mL
3.0-3.4 µm
5x1 mL
ECFP-F2-5K
PE Easy Calibration Kit, 5 peaks
2x106/mL
3.0-3.4 µm
5x1 mL
ECFP-F4-5K
PE-Cy5 Easy Calibration Kit, 5 peaks
2x106/mL
3.0-3.4 µm
5x1 mL
SPHERO™ Rainbow Linear Calibration Particles
Catalog No.
Description
Concentration
Nominal Size
Package Size
Price
RLP-30-5
Rainbow Linear Calibration Particles (For use in Linear Scale)
107/mL
3.0-3.4 µm
5 mL
SPHERO™ ACP Calibration Particles
Catalog No.
Description
Concentration
Nominal Size
Package Size
Price
ACP-30-5K
Allophycocyanin Calibration Particles, 6 peaks
107/mL
3.0-3.4 µm
2x5 mL
SPHERO™ Yellow Calibration Particles
Catalog No.
Description
Concentration
Nominal Size
Package Size
Price
YCP-70-5
Yellow Calibration Particles
107/mL
6.0-8.0 µm
5 mL