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Bio-Rad Flow Cytometry Calibration & Validation Reagents

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Flow Cytometry Absolute Count Standard™ is a suspension of microspheres that are approximately the size of human lymphocytes (7-9µm) and are presented as a known concentration. They are internally labeled with multiple fluorochromes and the combination of dyes allows the beads to be excited by a common argon laser (488nm) and emit in the three standard channels of a flow cytometer (FL1, FL2, FL3).

Protein Stablizer stabilizes antibodies and other proteins or biomolecules in solution. It may be used to stabilize ready-to-use pre-diluted standards and controls used in immunoassays.

Quantum™ FITC-5 MESF is comprised of five calibrated fluorescent populations, with different levels of FITC fluorescence intensity, and one blank population of uniform microspheres that are approximately the size of human lymphocytes (7-9µm). The excitation and emission spectra of the microspheres match those of cell samples labeled with FITC.

Quantum™ R-PE MESF Medium Level is comprised of four calibrated fluorescent populations, with different levels of RPE fluorescence intensity, and one blank population of uniform microspheres that are approximately the size of human lymphocytes (7-9µm). The excitation and emission spectra of the microspheres match those of cell samples labeled with RPE.

The levels of fluorescence intensity in the different kits (Low, Medium and High) are intended to span the intensity range of common cellular analyses. Medium level kits are used for many types of analyses, and nicely span the range of typical cell samples. Common analyses include those for many surface markers, including CD4 / CD8.

Quantum™ Simply Cellular IgG  is comprised of four coated populations and one blank population of uniform microspheres that are approximately the size of human lymphocytes (7-9µm). Each of the four sets is coated with goat-anti-mouse IgG (Fc-specific) with different calibrated Antibody Binding Capacities (ABC) for mouse monoclonal antibodies. The blank population has no specific binding capacity for mouse IgG.

When the bead populations are labeled in the same manner as the cells to be analyzed, they provide a means for constructinga QuickCal® calibration curve (ABC values vs. fluorescence intensity), from which samples may be "read". Cellular antigen expression may thus be quantitated in ABC units. The calibration curve is also a useful means for determininginstrument linearity.

RPE Reference Standard consists of one vial of microspheres that are uniform in both size and fluorescence and are approximately the size of human lymphocytes (7-9µm). The excitation and emission spectra of the microspheres match those of cell samples labeled with RPE.