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Miltenyi Biotec MACSplex Custom Cytokine Kits

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MACSPlex Cytokine Assays are designed for determining concentrations of up to 7 soluble analytes in a single sample. The analysis is based on MACSPlex Capture Beads, which display defined fluorescence properties and can be identified using standard flow cytometry techniques.

The MACSPlex Cytokine Basic Kit contains the supplementary components necessary to perform a MACSPlex Cytokine assay. It has to be combined with the MACSPlex Cytokine 12 Standard, human and the desired MACSPlex Cytokine Reagents Kits, human or with the MACSPlex Cytokine 10 Standard, mouse and the desired MACSPlex Cytokine Reagents Kits, mouse, respectively. Do not mix human and mouse specificities in one experiment. MACSPlex Cytokine Reagents Kits are designed for detection of one or maximal up to seven analytes of choice in a single sample.

MACSPlex Capture Beads within the single MACSPlex Cytokine Reagent Kits contain fluorescently labeled bead populations, coated with a specific antibody reacting with one of the analytes within the sample. MACSPlex Capture Beads from different reagents kits display different fluorescence intensities. Samples containing unknown levels of analytes are incubated with the antibody-coated MACSPlex Capture Beads, and analytes bind to the specific antibody. Each detection reagent contains APC-conjugated antibodies specific for one analyte and is also added. Consequently, sandwich complexes are formed between the MACSPlex Capture Bead, the analyte, and the detection reagent. These complexes can be analyzed based on the fluorescence characteristics of both the MACSPlex Capture Bead and the detection reagent. Standards of known quantities of given analytes are provided with the MACSPlex Cytokine Standards and are used for the quantification of the analytes within the unknown samples.

The analysis of MACSPlex Custom Cytokine Assays require a flow cytometer with a blue (e.g. 488 nm) and a red (e.g. 635 nm) laser, which are capable of detecting FITC, PE, and APC. In combination with the Express Modes of the MACSQuant® Analyzers the MACSPlex Custom Cytokine Assays are optimized for automated measurement of the beads. They simplify flow cytometric analysis via predefined experiment settings as well as acquisition and analysis templates. They apply an automated gating strategy to populations of interest that will be automatically adjusted for each data file individually to achieve optimal results.

Applications

MACSPlex Cytokine Reagent Kits, human have been developed for the flow cytometric detection of the soluble human cytokines GM-CSF, IFN-α, IFN-γ, IL-2, IL-4, IL-5, IL-6, IL-9, IL-10, IL-12p70, IL-17A, or TNF-α in a single sample. The kit has been optimized for use with serum, plasma, and cell culture supernatants.

MACSPlex Cytokine Reagent Kits, mouse have been developed for the flow cytometric detection of the soluble mouse cytokines GM-CSF, IFN-γ, IL-2, IL-4, IL-5, IL-10, IL-12p70, IL-17A, IL-23, or TNF-α in a single sample. The kit has been optimized for use with serum, plasma, and cell culture supernatants.