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Bangs Laboratories Affinity Ligand Coated Microspheres

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When circumstances call for the rapid development of microsphere reagents, particles coated with affinity binding proteins offer a ready solution. Affinity binding protocols are straightforward, typically involving a simple incubation of the affinity ligand coated spheres with the appropriate binding partner. Affinity proteins also provide an opportunity to optimally orient the ligand, as with the end-point immobilization of biotinylated oligonucleotides to streptavidin-coated microspheres, or the Fc-specific immobilization of antibodies via Protein A or G.

Particles coated with binding proteins also find significant use in purifications. Streptavidin-coated microspheres are often used in the isolation of biotinylated PCR amplicons. Protein A and Protein G microspheres are utilized for purification of antibodies and Fc-tagged fusion proteins, as well as for the recovery of immunoprecipitated Ab/Ag complexes.

Streptavidin

Our streptavidin and SuperAvidin™ coated microspheres offer simple and extremely stable binding of biotinylated molecules, such as antibodies, peptides, and oligonucleotides for use in diagnostics, screening, and affinity purifications. See TechNote 101, Affinity Ligand coated microspheres. Streptavidin coated microspheres are available in these standard volumes: 1mL, 2mL, 5mL, or 10mL. See also our BioMag® offerings.

Catalog CodeDescriptionProduct Data Sheet
CM01N Magnetic / Streptavidin PDS 721.pdf
CP01N Polymer / Streptavidin PDS 721.pdf
CP01F Fluorescent Polymer / Streptavidin PDS 721.pdf
CP01* Dyed Polymer / Streptavidin PDS 721.pdf
CS01N Steptavidin-Coated Silica PDS 702.pdf
PMS1N ProMag™ 1 Series • Streptavidin PDS 715.pdf
PMS1HC ProMag™ HC 1 Series • Streptavidin PDS 745.pdf
PMS3N ProMag™ 3 Series • Streptavidin PDS 715.pdf
PMS3HP ProMag™ HP 3 Series • Streptavidin PDS 743.pdf

* The final character of the catalog code denotes color: B = Blue, R = Red, O = Orange, K = Black, V = Purple, G = Green, F = Fluorescent, N=None.

Biotin

Our biotin-coated microspheres have been fully characterized in terms of their ability to bind free avidin, and therefore will require minimal optimization when determining the correct concentration of ligand to be bound. See TechNote 101, Affinity Ligand coated microspheres, for sample applications and procedures. See also our BioMag® offerings.

Catalog CodeDescriptionProduct Data Sheet
CP10N Polymeric / Biotin PDS 724.pdf

Protein A and Protein G

Protein A and Protein G are proteins that readily bind the Fc regions of many types of antibodies. For this reason, they are used extensively for affinity purification of antibodies from serum or cell culture supernatant, and for capture of immunoprecipitated protein complexes. When Protein A and Protein G microspheres are used as reagents in tests and assays, the immobilized antibodies may be crosslinked to the Protein A- or Protein G-coated support for long-term stability. See TechNote 101, Affinity Ligand coated microspheres. Protein A and Protein G coated microspheres are available in these standard volumes: 1mL, 2mL, 5mL, or 10mL. See also our BioMag® offerings.

Catalog CodeDescriptionProduct Data Sheet
CM02N Magnetic / Protein A PDS 722.pdf
CP02N Polymer  / Protein A PDS 722.pdf
PMG3N ProMag™ (Magnetic) / Protein G PDS 722.pdf