Bookmark with More

Miltenyi Biotec Viobility™ Fixable Dyes

Visit the website

The Viobility™ Fixable Dyes have been developed for the detection and discrimination of dead cells. These dyes react with the primary amine groups of proteins which can be found on the cell surface as well as intracellularly. The following reagents are available:

  • Viobility 405/452 Fixable Dye (Ex.: 405 nm, Em.: 452 nm) - DATA SHEET
  • Viobility 405/520 Fixable Dye (Ex.: 405 nm, Em.: 520 nm) - DATA SHEET
  • Viobility 488/520 Fixable Dye (Ex.: 488 nm, Em.: 520 nm) - DATA SHEET

The Viobility™ Fixable Dyes allow the discrimination between live and apoptotic or dead cells by flow cytometry. The dyes are suitable for both fixed and unfixed cells.

In contrast to viable cells, dead cells exhibit a compromised cell membrane allowing the Viobility Fixable Dyes to enter the cell and stain proteins within the cell. This results in a up to 50-fold brighter fluorescent staining and thus allows the discrimination between dead and viable cells by using a flow cytometer.Viobility Fixable Dyes show certain advantages over traditional dead cell–staining dyes like propidium iodide (PI):

  • non-toxic to cells
  • less spillover into other fluorescent channels
  • more flexibility in antibody panel design, as dyes are available for various excitation and emission wavelengths