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BioLegend Live Cell / Dead Cell Discrimination

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It is critical to understand the degree of cell death in any flow cytometry assay and exclude those cells from the analysis. BioLegend provides DNA dyes, Propidium Iodide and 7-AAD, that enter and stain dead cells, but are impermeable to live cells for rapid, cost-effective analysis of unfixed cells.

In cases where cell fixation is required, we now introduce fixable Zombie Aqua™ and Zombie Yellow™. Learn more about our tools for live/dead cell discrimination below.

 

Strategy Guide

Zombie Aqua™ is used prior to cell fixation. It may not work correctly if your population of cells are already fixed, which essentially renders all of them dead. Be sure to read the Zombie Aqua™ or Zombie Yellow™ protocols for details.