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Exbio FagoFlowEx® Kit

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The FagoFlowEx® Kit is intended for examination of phagocytic activity of granulocytes by measuring the respiratory (oxidative) burst after their stimulation with E. coli bacteria in human heparinized whole blood using flow cytometry.

Regulatory Status CE-IVD

 

FagoFlowEx® Kit contains following reagents:

1. ED7042-1

E.coli – 5 vials containing lyophilized E. coli bacteria, 1 vial is intended for stimulation of 20 tubes.

2. ED7042-2

DHR123 – 5 vials containing lyophilized dihydrorhodamine 123, 1 vial is intended for staining of 60 tubes.

3. ED7042-3

PMA – 5 vials containing lyophilized PMA (phorbol 12-myristate 13-acetate), 1 vial is intended for stimulation of 20 positive controls.

4. ED7042-4

Lysing Solution – 15 ml

General references

  • Heyworth P, Cross A, Curnutte J (2003) Chronic granulomatous disease. Curr Opin Immunol 15(5): 578-84
  • Winkelstein J, Marino M, Johnston R et al (2000) Chronic granulomatous disease. Report on a national registry of 368 patients. Medicine (Baltimore) 79 (3):155-69
  • Sawyer DW, Donowitz GR, Mandell GL (1989) Polymorphonuclear neutrophils: An effective antimicrobial force. Rev Infect Dis 11: 1532-44

Example Data

 
Fago pacienti
Fig. 1.


Fig. 1. Examples of data obtained with FagoFlowEx Kit.