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Bio-Rad Mouse Seroblock FcR

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Mouse Seroblock FcR is a rat antibody that recognises mouse CD16 and CD32, cell surface proteins also known as FcRγIII and FcR&gamma:II, respectively.

The function of these proteins is to bind IgG molecules via their Fc regions as part of the adaptive immune response. CD16 and/or CD32 are expressed by a wide variety of cells, including monocytes, macrophages, B lymphocytes, granulocytes, NK cells, dendritic cells and some activated T lymphocytes.

The expression of CD16/CD32 antigens can lead to non-specific binding of test monoclonal antibodies in staining procedures, resulting in “high background” staining on a wide range of cells. This non-specific binding may be blocked by pre-incubation of target cells with BUF041A, resulting in clearer staining. For direct analysis of CD16/32 expression this antibody is also available conjugated directly to FITC (MCA2305F), AlexaFluor488 (MCA2305A488), AlexaFluor647 (MCA2305A647 ) and RPE (MCA2305PE ).

Applications of mouse Seroblock FcR

Mouse Seroblock FcR has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Where Mouse Seroblock FcR has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Please view the datasheet pdf for suggested dilutions.

Instructions for Use

In order to reduce Fc-receptor mediated binding of test antibodies the following procedure is recommended:-

1) Incubate the cell suspension (1 x 106 cells in 100ul) with 1ug of BUF041A (1ul of undiluted reagent, or 10ul of a 1/10 dilution) for 5-10 minutes.

2) Add test antibody according to manufacturers instructions – Do not wash BUF041A off the cells. BUF041A is suitable for use in conjunction with test antibodies from any manufacturer or with in-house antibodies.*

3) Proceed with staining as usual.

* Care is needed in the design of experiments utilizing unconjugated anti-mouse antibodies, to ensure that the secondary antibody being used does not cross-react with SeroBlock FcR. Bio-Rad supply a range of isotype specific anti-mouse immunoglobulin antibodies that may be useful for this purpose.