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Bio-Rad Human Seroblock

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Human Fc receptors are expressed on a variety of immune cell types including monocytes, macrophages, B cells, granulocytes and dendritic cells.

Cells that express Fc receptors can give false positive immunofluorescent staining due to the Fc receptors binding of Ig. Human SeroBlock is designed to prevent such non-specific staining without interfering with appropriate target staining. Human SeroBlock is compatible with use of anti-human antibodies targeting Fc receptors in flow cytometry.

Applications of Human Seroblock

Human Seroblock has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Where Human Seroblock has not been tested for use in a particular technique, this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Please view the datasheet pdf for suggested dilutions.

Instructions For Use

In order to reduce Fc-receptor mediated binding of test antibodies the following procedure is recommended:-

1) Add 5 ul of Human SeroBlock per 100ul cell suspension for 5-10 minutes at room temperature.

2) Add test antibody according to manufacturers instructions – Do not wash Human SeroBlock off the cells. Human SeroBlock is suitable for use in conjunction with test antibodies from any manufacturer or with in-house antibodies.*Human SeroBlock is also compatible with flow cytometric analysis of human Fc receptors.

3) Proceed with staining as usual.