Bookmark with More

IQ Products Fetal Cell Count™ Kit

Visit the website

Diagnosis of Fetomaternal Hemorrhage (FMH)

Complete assay for routine diagnosis of Fetomaternal Hemorrhage using Anti-CA and Anti-HbF

Applications

  • Determination of Fetomaternal Hemorrhage
  • Pregnancy with suspected RhD incompatibilities
  • Abdominal trauma
  • Recognize fetal cells in maternal Thalaessemia circulation

Features

  • Unique quantification of Fetomaternal Hemorrhage by flow cytometry
  • Complete assay for routine diagnosis of Fetomaternal Hemorrhage
  • Detection as low as 0.02% fetal cells in maternal blood
  • Detection of fetal haemoglobin (HbF) and Carbonic Anhydrase (CA)
  • Simple and easy handling procedure
  • IVD/CE registered

Introduction

Detection and quantification of fetal red blood cells (fRBCs) in maternal blood samples is essential for obstetrical management. Measurement of fRBCs is critical as the extent of Fetomaternal Hemorrhage (FMH), the transplacental passage of fRBCs into the maternal circulation, has consequences for further treatment of mother and child. Frequency and size of FMH is directly influenced by complications in abdominal trauma, suspected placental injury or after a caesarean section. Severe FMH may lead to intra-uterine death. In case of antigen incompatibility between mother and child FMH may result in respiratory problems or anaemia, like Haemolytic Disease of the Newborn.

Adult RBC’s contains a population of HbF (F-cells), which can be differ between 0 and 14%. The F-cells can give a positive result in the Kleihauer-Betke acid-elution test or single HbF flow cytometry test. These F-cells may be the result of physiological variations during pregnancy or traits such as thalassemia, sickle cell anaemia or hereditary persistence of fetal haemoglobin.

The detection (and thus enumeration) of fRBCs is used to calculate the extent of FMH, either in case of trauma with suspected placental injury or in the situation of a RhD incompatibility between the fetus and the mother. The amount of fRBCs is a measure for the prevention of hemolytic disease of the newborn using (prophylactic) anti-D therapy.

Principle of the Fetal Cell Count™ Kit

The Fetal Cell Count™ Kit assay is a flow cytometry product. The assay is based on a  patented combination of two antibodies. One is directed against HbF while the second is specific for carbonic anhydrase (CA), an enzyme present in adult RBCs and, at very low detectable level, in late pregnancy stage.
•    Results within 90 minutes
•    Precise and accurate detection of fRBCs
•    Intracellular detection of HbF and CA
•    Distinguishes between fRBCs and maternal F-cells and RBCs, it eliminates subjective interpretations an empiric signal cutoffs