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Bio-Rad CytoTrack™ Cell Proliferation Assays

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CytoTrack™ cell proliferation assays are designed to efficiently stain live cells for excellent resolution of each cell division generation.

Using a proprietary chemistry, the dye reacts with primary amines and is retained in the cell with minimal efflux, allowing up to 10 cell divisions to be resolved.

As cell division occurs, the dye is successively halved and the different generations can be determined based on the decrease in fluorescence intensities.

CFDA-SE, the most commonly used reagent for monitoring cell proliferation, is difficult to use in multicolor experiments because it has the same spectra as green fluorescent protein and the most frequently used fluorophore, fluorescein.

Unlike CFDA-SE and other popular cell proliferation assays that are available in only one color, Bio-Rad’s CytoTrack Assays are available in four different emission wavelengths ranging from blue to far red. This allows researchers to choose the one most suitable for their multicolor flow cytometric experiment.

The CytoTrack™ Assay dye reacts with primary amines using a proprietary chemistry that provides effective labeling of cells without the large efflux usually seen with CFDA-SE.

“This unique feature allows researchers to easily visualize up to 10 cell divisions, making it ideal for those interested in studying the induction and inhibition of cell division in any in vitro experimental model,” said Mary Thao, Bio-Rad Product Manager in the Gene Expression Division.

CytoTrack™ Assays come in easy-to-use vials with material sufficient for 50 experiments. The dye can be added directly into the culture medium, allowing researchers to save time.

Bio-Rad offers additional reagents and consumables to complete each step of flow cytometry experiments, such as cell viability assays, primary antibodies, and antibody labeling kits.

 

CytoTrack CFDASE Graph

Cell division monitoring using CytoTrack™ Green 511/525 (A) and compared to CFDA-SE (B). CytoTrack™ Green shows a reduced efflux between generation 0 and 1.

Key Benefits of CytoTrack Cell Proliferation Dyes

  • Fix and permeabilize cells stained with CytoTrack™ dyes for analysis of intracellular targets— compatible with standard formaldehyde-containing fixatives and saponin-based permeabilization buffers
  • Combine CytoTrack™ assays with fluorescent proteins or antibody-labeled fluorophores for multicolor cell analysis
  • Save time by using the cell proliferation assay directly with your cell culture media
  • Detect up to 10 cell divisions

Related Products

For convenient CFSE staining, we offer an additional cell proliferation assay featuring CFDA-SE dye (135-1201) to visualize up to 8 cell divisions.

Additional Products for Flow Cytometry

  • Cell viability assays for flow cytometry — ready-to-use dead cell exclusion assays
  • Antibody labeling kits — label your antibody of interest in 2 easy steps without a purification column
  • Primary antibodies — use high-quality monoclonal and polyclonal primary antibodies for applications ranging from stem cell, immunology, cell biology to veterinary research

More Information

Learn more about the features of the S3™ cell sorter and its associated consumables and accessories.