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Vybrant® Phagocytosis Assay Kit

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The Vybrant® Phagocytosis Assay Kit provides a way for researchers to observe and quantitate the process of phagocytosis in human polynuclear cells and mouse macrophages by following the internalization of a foreign particle—in this case killed E. coli (K-12 strain) cells that have been labeled with the fluorescent dye fluorescein.

In addition to the lyophilized E. coli BioParticles® component, the kit contains a trypan blue solution (to quench the fluorescence from particles that were not internalized) as well as step-by-step instructions for performing this phagocytosis assay in a fluorescence microplate reader. The methodology used with this kit has been developed using an adherent murine macrophage cell line (J774); however, by modifying the cell culture conditions, researchers can adapt this phagocytosis assay to other adherent cell lines.

Vybrant® Phagocytosis Assay Kit Specifications:

• Particles are fluorescein-labeled E. coli (K-12)
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