Bookmark with More

T-cell/Antigen Presenting Cell Conjugation

Visit the website

Topic: Studies of T-cell/Antigen Presenting Cell Conjugation Using Imaging Flow Cytometry
Speaker: Haley R. Pugsley, Ph.D., Application Specialist, Merck Millipore
Date: May 15, 2013
Time: 12:00 pm - 1:00 pm (EDT)

Imaging flow cytometry combines the speed, sensitivity, and phenotyping abilities of flow cytometry with the detailed imagery and functional insights of microscopy. This unique combination enables a broad range of applications that would be impossible using either technique alone. This seminar will explore two studies of the T-cell/Antigen Presenting Cell (APC) Immunological Synapse performed on FlowSight® and ImageStream®X Mark II imaging flow cytometers.

In the first study we will demonstrate image-based parameters that were used to assess the frequency of T-cell/APC conjugates with an organized immunological synapse in an objective and statistically significant manner. In the second study we evaluate the specific location of the adhesion and signaling molecules LFA-1 and Lck within the immunological synapse complex in T-cells when presented with SEB, as well as the activation of the T cells in contact with APC by measuring the nuclear localization of NFkB in the T cell.