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Essential Instrument and Experimental Design Considerations

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This webinar is designed to discuss current trends of flow cytometry technologies with regard to a variety of experimental processes in the laboratory.

Using a complex technology such as flow cytometry effectively demands attention to a number of instrument and experimental design issues. Fortunately, there are many resources available to assist users in understanding the limitations and opportunities of the technology.

Drs. J. Paul Robinson (Purdue University) and John P. Nolan (La Jolla Bioengineering Institute) will discuss some of the calibration and standardization approaches that will ensure that the experimental data obtained is accurate and readily interpreted by other researchers. In addition, expanding assays from a few tubes to large plate arrays demands a rethinking of the experimental design, execution and data analysis.

Finally, with the increased use of cell sorting, many users may not be aware of the important issues involved in appropriate and acceptable safety involved in sorting viable human cells, or cells containing infectious agents. Essential elements of each of these topics will be discussed and additional resources available to flow cytometry users will be highlighted.

Drs. Robinson and Nolan will answer your questions live during the webinar so be sure to attend and get answers from the experts!

What you will learn in this webinar:

  • Essential concepts in flow cytometry instrument and assay calibration and standardization
  • Crucial considerations for expanding basic flow assays to larger scale and higher throughput
  • Cell sorting safety practices
  • An overview of Current Protocols in Cytometry coverage across many different fields of science

Who should attend:

  • Researchers who need to develop greater expertise in using flow cytometry
  • Faculty interested in evaluating current flow cytometry practice and opportunities
  • Technical staff who are new or experienced operators

More about the Presenters:

Dr. J. Paul Robinson is the SVM Professor of Cytomics and Professor of Biomedical Engineering at Purdue University

Dr. J. Paul Robinson is the current SVM Professor of Cytomics and Professor of Biomedical Engineering at Purdue University. He is a past president of the International Society for Advancement of Cytometry (ISAC) and has been working in the field for over 30 years. He is the managing editor of Current Protocols in Cytometry.

Dr. John P. Nolan is the President of the  International Society for Advancement of Cytometry

Dr. John P. Nolan leads a group developing cytometry instrumentation and applications at the La Jolla Bioengineering Institute. He is on the Editorial Boards of Cytometry and Current Protocols in Cytometry, and is the current President of ISAC.