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Bio-Rad Fluorescence and compensation in flow cytometry

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Why fluorophores need compensating and how to avoid unnecessary compensation

Fluorophores accept light at a specific wavelength and re-emit it at a higher wavelength, giving each fluorophore an excitation and emission wavelength. Although there is a peak or maximal emission the range is often several hundred nanometers. This can cause problems where unwanted light enters several detectors giving false positives.

Compensation is required to remove this unwanted fluorescence. Careful choice of fluorophores can reduce the amount of compensation needed

The webinar is recommended to novices and researchers with limited experience of flow cytometry. The webinar covered:

  • Basic fluorescence principles
  • What is compensation and why you need to apply it
  • Compensation controls
  • How to avoid compensation in small panels
  • Common rules to help build more complex panels