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Secondary antibodies reference guide

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Secondary antibodies recognize an antibody as their target antigen, usually referred to as the primary antibody which in turn is bound to its antigen or protein of interest.

Unlike anti-idiotypic antibodies which bind specifically to the antigen-binding domain, secondary antibodies are specific to the conserved regions of an antibody which are constant within a particular class of antibody such as human IgG or mouse IgM.

Secondary antibodies are therefore able to bind to ranges of antibody clones making them very effective research tools.

Here we describe the applications of secondary antibodies and the considerations needed in their selection.