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Duke Human Vaccine Institute Flow Cytometry Facility

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Shared Resource Summary

The DHVI Research Flow Cytometry Facility, led by John Whitesides, serves the analytical and cell sorting needs of the DHVI as well as support for researchers throughout the Duke Community.

The Flow Cytometry Facility offers state-of-the-art cytometric support to DHVI investigators in Basic, Developmental, and Clinical Research.  The Core consists of a Non-BSL-3 Flow Cytometry Facility and a BSL-3 Flow Cytometry Facility, which assist investigators in developing assays for measuring human immune responses to vaccines and infectious agents providing critical information about cell surface structure, identification, internal components and cell physiology. 

The Flow Cytometry Facility offers several cytometric services including BSL-1 through 3 live cell sorting, phenotypic acquisition, FRET (Fluorescent Resonance Energy Transfer), DNA cell cycle analysis and intracellular marker analysis.  The analytical and sorting abilities exceed 8 simultaneous parameters and enable researchers to define subpopulations based on cell surface morphology as well as size and complexity. 
 
The DHVI Research Cytometry Facility is partially supported by the NIH and conforms with guidelines established to ensure that the facility has accessibility to the Duke Community.  Our facilities’s goals are to supply our researchers with critical information about disease models that lead to direct applications related to clinical trials and human diseases and infections.  Our facility also aids in the discovery of key elements in stem cell research, cardiovascular disease, bone marrow transplantation and immune reconstitution.
 
Personnel/Contact Information:

Director: John F. Whitesides, PhD
Office Phone: (919) 684-4895
Lab Phone: (919) 684-4130
Fax: (919) 684-3282
Email: jwhtsds@duke.edu

Manager: Patti McDermott
Office Phone: (919) 684-4130
Lab Phone: (919) 684-4130
Fax: (919) 684-2161
Email: pmcderm@duke.edu

 

Location: 4113 MSRBII, DUMC Box 103020, Durham, NC 27710

              1029 GHRB, DUMC Box 103020, Durham, NC 27710