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University of York Imaging & Cytometry Facility

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Imaging and Cytometry laboratory

The Imaging and Cytometry Laboratory provides a facility committed to bringing state of the art technology to researchers with varying levels of expertise. A significant effort is devoted to training scientists in imaging and cytometry techniques to advance their projects. The facility is available to both internal and external users.

Our staff provide fully assisted technical support for the infrequent user and the facility is an excellent equipment resource for the experienced user.

 

Equipment

Techniques

Flow Cytometer

  • Reference site for Beckman Coulter
  • 2 CyAn analysers with 3 lasers, 11 detectors
  • 2 MoFlo high-speed, sterile cell sorter (80k/s with final concentration of ~1 x 106/s)
  • Apogee flow system A40
  • Rare cell sorting
  • Simultaneous cell cycle and receptor analysis
  • FRET
  • Small particle analysis
Confocal microscopy
  • Multiphoton Zeiss LSM 510 NLO meta4 laser (7-line)
  • Zeiss Meta with spectral capability on invert fully motorised microscope
  • Zeiss Meta on a fully motorised upright microscope
  • Spinning disk system for high speed and live-cell
  • Live cell imaging chamber
  • Upright and invert microscopes
  • Spectral analysis and unmixing
  • Multicolour work
  • FRET
  • FRAP
  • FLIM
  • Live-cell time
  • Deep tissue imaging

Electron microscopy

  • TEM with CCD
  • Scanning EM with cryosystem
  • Sub-nanometre resolution
  • Immunogold studies
  • Tilting stage for 3-D
  • Cryo-SEM service

Laser capture / microdissection microscopy

  • PALM system with live cell capability
  • Rare cell isolation
Image analysis software packages Off-line analysis

 

Peripheral equipment and histology

Head of Imaging and Cytometry Laboratory

Dr Peter O'Toole Dr Peter O'Toole
Technology Facility
Department of Biology (Area 15)
University of York
Wentworth Way
York YO10 5DD
UK

Office: +44 (0)1904 32 8722
Fax: +44 (0)1904 32 8804
Email: pot1@york.ac.uk