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University of Surrey Flow Cytometry & Bioimaging (BIAFC)

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The University of Surrey Bioimaging and Flow Cytometry Core Facility supports the imaging and flow cytometry requirements of staff and students at the University of Surrey, as well as to outside academic and industrial institutions upon request. We have a wide research user base, with particular interests in cell biology, immunology, bacteriology, virology, cancer biology, and cell death. 

We provide full training and any assistance required in flow cytometry and confocal microscopy. We also offer a cell sorting service. Please direct requests for equipment booking and training to Dr Robert J Francis, Facility Manager r.francis@surrey.ac.uk.

BIAFC costing structure

Instruments


Flow Cytometry

The instruments within the core facility allow for multi-parameter analysis of your cells, including eukaryotic and prokaryotic cells which can either be alive or fixed. It is also possible to sort your cells into separate populations based on their characteristics as shown by fluorescent indicators. See link below for further details on the capabilities of the instruments within the facility to enable fluorochrome choice.

The facility is equipped with the following flow cytometers and cell sorter:

  1. BD Biosciences FACS ARIA (3-laser, 9 colour), Windows XP computer with FACS DIVA v5.0
  2. BD Biosciences FACS Canto (2-laser, 6-colour), Windows XP computer with FACS DIVA v5.0
  3. BD Biosciences FACS Scan (1-laser, 3 colour), G4 PowerMac System 9.2, with CellQuest v3.3
  4. Beckman Coulter Epics XL (1-laser, 3 colour), Windows 98 PC with EXPO32

Instrument Specifications


Bioimaging

The instruments that are available allow for multi-dimensional analysis of your cells including eukaryotic and prokaryotic cells. Analysis is based on fluorescence and brightfield imagery, and samples can either be alive or fixed.  See link below for further details on the capabilities of the instruments within the facility to enable fluorochrome choice and experimental design.

The facility is equipped with the following microscopes:

  1. Zeiss LSM510 VIS META Confocal Microscope (on an Axiovert 200M microscope)
  2. ***New for 2013*** Nikon A1M Confocal Microscope and DS-Qi1 Widefield Camera (on an Eclipse Ti-E Microscope)

Instrument Specifications

Contact Us

Academic Lead

Professor Graham Stewart

Professor of Molecular Bacteriology

01483 68 6423

g.stewart@surrey.ac.uk

 

Support Staff

Dr Robert Francis

Research Fellow in Bioimaging and Flow Cytometry (Facility Manager)

01483 68 6428

r.francis@surrey.ac.uk

 

Mrs Rebecca Holland

Technical Support for Confocal Microscopes

01483 68 6387

r.holland@surrey.ac.uk