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BitesizeBio Flow Cytometry Channel

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ABOUT BITESIZE BIO

'Being a researcher is tough. So much to learn, and so little time. As well as all of the technical know-how you’ve got to keep up on, you need skills in writing, communication, administration, management, and…. need we go on?

Bitesize Bio is the place where you can learn, share and discuss all of these skills to help you become a better, more rounded and hopefully less stressed, scientist. Our articles and webinars are deliberately kept short (“bitesize” – get it?) so that you can take all of this stuff on board easily and without taking up too much of your time.

Please dive in and enjoy…'

Sweetie-Jars

Article

Pick ‘n’ Mix? The Guide to the ‘Sweet’ Shop of Flow Cytometers

  So having read our article on how a cytometer works, surely the next question is ‘what’s the right flow cytometer for me?!’ Basic Components of Flow Cytometers We know that at their most basic level, cytometers are made up of 3 main components: A fluidics system to transport and focus the cells past an [...]

Read more   July 05, 2013 by

Chromatin-Fragmentation

Article

Flow Cytometric Apoptosis Assays to Characterize Cell Death

Apoptosis, often called programmed cell death, is a carefully regulated process that is part of normal development and homeostasis. Apoptosis is morphologically and biochemically distinct from necrosis, which is conversely called accidental cell death. Dysregulation of apoptosis is implicated in disease states such as cancer, autoimmune disease and degenerative conditions. An orderly sequence of events [...]

Read more   July 03, 2013 by

Mixing-Colours

Article

An Introduction to Spectral Overlap and Compensation Protocols in Flow Cytometry

It strikes fear into the hearts of new cytometrists. Compensation. More fights have started over the proper way to compensate at meetings. This article will strive to shed some light on the principles of compensation, and equip you with the tools necessary to achieve compensation mastery for your research experiments. Compensation is one of the [...]

Read more   May 06, 2013 by

Scattering-of-Light

Article

Basic Parameters Measured by a Flow Cytometer: What is Scattered Light and Absolute Fluorescence?

Now that you have a basic understanding of the inner workings of a flow cytometer, it’s important to grasp the types of measurements that are being made and, perhaps more importantly, what measurements are NOT being made. For simplicity’s sake, we’re going to frame this discussion in terms of a classical flow cytometer; think of [...]

Read more   April 01, 2013 by

Sorting large cellular complexes by flow cytometry

Article

Sorting Large Cells and Materials by Flow Cytometry

Flow cytometers and cell sorters were designed with blood cells in mind. This means that commercial cell sorters are optimized for sorting cells typically smaller than about 20 µm in diameter. However, it turns out that many cell types, including those of mammals, are larger than 20 µm. So what are your options if you [...]

Read more   March 04, 2013 by

ink-jet-cells

Article

Cell Sorting by Flow Cytometry: How do You do That Then?

Flow cytometry is an extremely powerful technology that can be used to look at multiple fluorescence measurements on thousands of cells per second. This allows multiple subsets of cells within a heterogeneous population to be identified and quantitated and this is what analytical flow cytometers are great at. However, once you have identified a population [...]

Read more   February 04, 2013 by

Tails-from-the-Nozzle-Bank

Article

Evolution Flow: The Historical Background of Flow Cytometry

For those of you who print out the results that you have acquired from the Flow Cytometer to stick in your lab book, did you know that inkjet printers and cytometers have a shared history? Flow Cytometry is less than 50 years old and machines today still use some of the same principles as the [...]

Read more   January 28, 2013 by

The Magic Box

Article

How a Flow Cytometer Works: A Look Inside the Magic Box

A flow cytometer is a device used to illuminate objects and capture and quantitate light emitting from these objects.  The “objects” are normally single cells dispersed in a medium, but could very well be polystyrene beads, cell fragments or debris, or even large molecules. So, what’s in the box? Using your highly tuned powers of deduction, you [...]

Read more   January 21, 2013 by

Unlock your dreams

Article

Remote Cytometry: Help from beyond!

The idea of accessing one computer from another is long established. Unfortunately we often have visions of hackers sneaking in and stealing our data when we have most to lose. However, this type of technology can aid us in a lot of applications and to those of us who work in cytometry the benefits are (somewhat) clear. No more ‘fail’ moments Many researchers know the dread of [...]

Read more   January 14, 2013 by

DD-001-Image-Scaled1 2

Article

Cell Cycle Analysis by Flow Cytometry: Flowing your Way through Life’s Cycle

Over the past few decades the mammalian cell cycle has been well documented. Although there are lots of checkpoints as cells move through the cycle, we can very simply divide the cell cycle into three stages according to the DNA content in the nucleus. When cells are either quiescent or not dividing they have the [...]

Read more   January 07, 2013 by