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Flow Cytometry - A Basic Introduction (MG Ormerod)

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'This site is devoted to building a connected community around Flow Cytometry. It is intended to serve as a resource for information, protocols, e-Learning and resources. We have chosen the wiki format to allow all users to contribute their experience and knowledge to build a vibrant community.'

What is a Wiki?

Almost every page is editable by all users of our site. We rely on our community members to help add and maintain the content of the site. We hope that you will participate. Everyone has knowledge they can share to improve the quality and usefulness of flow cytometry around the world. Please contribute by adding a comment, posting a sample analysis, correcting an error or adding a new topic. Click here to find out more about a wiki and how to add and edit pages.

Even if you are not ready to contribute yet, please feel free to browse our site. We have a lot of interesting things, including background information, sample data sets, reference material, and more.

Table of Contents




Chapter 1 Introduction 
Chapter 2 The Flow Cytometer 
Chapter 3 Fluorescence & Fluorochromes  
Chapter 4 Data Analysis 
Chapter 5 Immunofluorescence and colour compensation 
Chapter 6 DNA Analysis 
Chapter 7 Some Clinical Applications 
Chapter 8 Cell Proliferation 
Chapter 9 Cell Death, including apoptosis 
Chapter 10 Some Other Applications 
Appendix 1 Abbreviations 
Appendix 2 Glossary 
Appendix 3 Useful Websites 
Appendix 4 Safety 

You can also find a static web version of the book by clicking here. The static version is maintained exclusively by Dr. Michael Ormerod and contains all of the information in the book.

A bonus feature of the online version of the book is that most of the figures have links to the data file from which they were derived. If you have downloaded the free FCS Express Reader, you can open the analysis layout that was used to create each figure. In some cases, extra information about the figure is supplied, including other data files. The reader can experiment with the data, changing the position of the gates, markers, quadrants etc.

To get an overview of the book and the intended audience, please read the Preface.

We hope that you enjoy this new venture and look forward to hearing any comments.

Michael Ormerod
David Novo