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A History of Fluors and Cytometers

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Flow cytometry is a useful application used in countless labs for the phenotyping of immune cells. When discussing flow cytometers, fluorophore development is inextricably intertwined. As more and more fluorophores were generated, more complex cytometers were invented to increase multicolor flow cytometry options.

Decades ago, multicolor flow experiments were capped at 2 or 3 colors; today we're up to 18 or 20. Take a look at our timeline to learn how the flow cytometers and your favorite fluorophores came about.



1. Alivisatos, A.P. 1996. Science. 271:933. JSTOR
2. Bendall, S.C. et al. 2012. Trends Immunol. 33:323. Pubmed
3. Panchuk-Voloshina, N. et al. 1999. J. Histochem. Cytochem. 47:1179. Pubmed
4. Shapiro, H. Practical Flow Cytometry, 4th ed. Hoboken: John Wiley & Sons, Inc. 2003. Wiley