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Category 'Antibodies'
Status: Unanswered
Views: 96

Ki67 antibody conjugated for flow cytometry for CD3+ lymphocytes

fiona cunningham (Author)

Monday, February 18, 2019 9:13 AM

Dear all,

kindly could you recommend which clone of Ki67 antibody (conjugated for flow cytometry) is good for determining the proliferation of CD3+ Lymphocytes please?

I am just starting so I am simply going through journals but can't be sure which is best  ie anyone find KI67-PE (B56) BD Biosciences any good ?  recommendations for a very effective clone are really appreciated. 
Thanks

Fiona.

Marcel Trefny

Monday, February 18, 2019 9:41 AM

Hi Fiona,

 

B56 in BV421 from BD has worked well for us. Be aware, the most imporant thing is the fixation and permablization to stain for nuclear markers. We use eBiosciences Fixation/Permeablization kit for this.

 

Best

Marcel