WORK-FLOW

Education

Educational Program

PlansIncludesAccess
Access+. Flow Cytometry Essentials
. Cytometry Select (with future releases)
. FlowHub (with quarterly updates)
. FlowEval (with future updates)
Register

For additional information on pricing, payment, and bulk discounts, check out our FAQ (“What is the cost of a license and how can I pay?”).

If you are wondering why you should choose our program, consult our FAQ (“Why use WORK-FLOW’s Educational Program?”).

Flow Cytometry EssentialsTM is an on-demand, streamlined, brand-neutral training program, delivered as subtitled video-based micro-content and simulation.

Course Details

Modern and cost-effective, Flow Cytometry EssentialsTM (FCE) eliminates the tedious and time-consuming theoretical classes usually employed by core facilities and guarantees that all users receive the same quality information on common concepts and practical considerations before attending an instrument-specific session.

Approved users can create their own account and immediately start taking the course anywhere and at their own pace.
Beginners can stop and rewind the videos to fully understand the concepts or revisit the illustrations. Advanced users needing a refresher or required to take this course will make efficient use of their time thanks to the optimized delivery.

A total of 75 quiz questions, evenly allotted at the end of each section, help ascertain knowledge validation prior to the obtention of a certificate of completion.
An extensive glossary and access to the FlowHub, our resources platform, are available to complement the learning process.

1 – Principles & Instrumentation

  1. What is Flow Cytometry?
  2. Common Applications
  3. Fluorescence – Light Spectrum
  4. Fluorescence – Absorption & Emission
  5. Fluorescence – Fluorochrome Classes
  6. Fluorescence – Fluorochrome Properties
  7. Fluidics – Flow
  8. Fluidics – Pressure & Flow Rate
  9. Fluidics – Acoustic-Assisted Focusing
  10. Optics – Excitation
  11. Optics – Light Collection
  12. Optics – Photodetection
  13. Electronics – Amplification & Conversion
  14. Electronics – Voltage Pulse
  15. Electronics – Time Delay
  16. Electronics – Digitization & Binning
  17. Electronics – FCS File
  18. Section Summary

2 – Reagents & Panel Design

  1. Sample Requirements
  2. Cellular Markers and Dyes
  3. Fluorescence Detection
  4. Fluorochrome Selection
  5. Antigen Classification
  6. Antibody Titration
  7. Dead Cell Staining
  8. Exclusion / Dump Channel
  9. Spectral Overlap
  10. Compensation Controls
  11. Compensation
  12. Spectral Overlap Reduction
  13. Spillover Spreading
  14. Spillover Spreading Matrix
  15. Autofluorescence
  16. Fc-Mediated Binding
  17. Fluorescence Minus One Controls
  18. Biological Controls
  19. Replicates
  20. Section Summary

3 – Acquisition & Measurements

  1. Plots & Gates
  2. Scaling
  3. Acquisition Speed
  4. Event Cycling
  5. Scatter Setup
  6. Threshold & Trigger
  7. Aggregate Discrimination
  8. Dead Cell Exclusion
  9. Fluorescent Parameter Setup
  10. Compensation
  11. Data Recording
  12. Data Exporting
  13. Assay Setup Validation
  14. Instrument Cleaning
  15. Section Summary

4 – Analysis (with Interactive Tools)

  1. Matrix Inspection
  2. Data Display – Histograms & Plots
  3. Data Display – Gates, Regions, Quadrants
  4. Data Display – Gating
  5. Biexponential Scaling
  6. Data Cleaning – Time Gating
  7. Data Cleaning – Debris Exclusion
  8. Data Cleaning – Aggregates & Dead Cells
  9. Data Cleaning – Autofluorescence
  10. Data Cleaning – Antibody Aggregates
  11. Data Cleaning – Impact
  12. Gate Validation – FMO Controls
  13. Gate Validation – Backgating/Color Gating
  14. Cell Cycle – One-color DNA Analysis
  15. Cell Cycle – Multi-color DNA Analysis
  16. Section Summary

Interactive Tools

  1. Matrices
  2. Plotting
  3. Gating
  4. Scaling
  5. Cleaning Impact
  6. Controls
  7. DNA Analysis

5 – Statistics

  1. Number of Events to Record
  2. Descriptive Statistics – Distribution
  3. Descriptive Statistics – Central Tendency
  4. Descriptive Statistics – Dispersion
  5. Descriptive Statistics – Data Normalization
  6. Descriptive Statistics – Fold Change
  7. Inferential Statistics – Statistical Test
  8. Inferential Statistics – Statistical Power
  9. Inferential Statistics – Hypotheses
  10. Inferential Statistics – Significance Level
  11. Inferential Statistics – Statistical Errors
  12. Inferential Statistics – Probability Value
  13. Student’s t-test – T-distribution & Freedom
  14. Student’s t-test – Formula
  15. Student’s t-test – Real-Life Example pt1
  16. Student’s t-test – Real-Life Example pt2
  17. Section Summary

Preview

Access Instructions

  • Already registered? Click LOGIN
  • Group leader? Access the User Management Dashboard (instructions)

Cytometry SelectTM is a series of cytometry courses on advanced, specialty topics.

CyS1 – Cell Sorting

Course Outline

  1. What is Cell Sorting? | Process Overview
  2. Nozzle Size & Sheath Pressure | Droplet Generation
  3. Droplet Breakoff
  4. Stream Charging & Deflection
  5. Collection
  6. Instrument Optimization | Drop Delay
  7. Acquisition & Gating
  8. Conflicts
  9. Sort Decisions
  10. Sorting
  11. Post-Sort – Purity, Recovery & Yield
  12. Process Stressors | Sample Quality
  13. Temperature & Pressure | Sample Media & Sheath
  14. Collection Media | Instrument Variables
  15. Sort Performance | Sort Quality
  16. Collection Issues | Contamination
  17. Single-Cell Deposition
  18. Biosafety | Summary
  19. Quiz (20 questions)

CyS2 – Full-Spectrum Flow Cytometry + Panel Optimization

Course Outline

  1. What is Full-Spectrum Flow Cytometry?
  2. Full-Spectrum vs. Conventional
  3. Spectral Emission Signature
  4. Signature Comparison
  5. Signature Alterations
  6. Reference Controls
  7. Spectral Reference Library
  8. Instrument Settings
  9. Acquisition
  10. Spectral Profile & Unmixing
  11. Autofluorescence Management
  12. Unmixing Errors
  13. Analysis
  14. Panel Optimization – Introduction
  15. Panel Optimization – Design
  16. Panel Optimization – Testing
  17. Panel Optimization – Evaluation & Remediation
  18. Summary
  19. Quiz (20 questions)

Access Instructions

  • Already registered? Click LOGIN
  • Group leader? Access the User Management Dashboard (instructions)

FlowHubTM is a proprietary support platform that centralizes resources and information to efficiently deliver knowledge, leading users from experimental design to publication.

Contents

This cytometrist’s companion guide is a combination of original material, hosted information, and external references, totaling more than 720 documents and links. A convenient tool perfect for continuing education, time-saving guidance, or exploration into new applications.

Preview

Access

  • Already registered? Click LOGIN

FlowEvalTM is a proprietary flow cytometry knowledge assessment platform.

Contents

FlowEval is a comprehensive evaluation and preparation resource designed to help test your knowledge whether you are studying for an exam or are simply interested in strengthening your understanding.

Key Features:
• Extensive Question Bank: Access a wide range of flow cytometry questions
• Detailed Explanations: Understand the “why” behind the answers of more complex questions
• Realistic Practice Exams: Simulate exam conditions with full-length practice tests
• Access Anywhere: Study on-the-go with our responsive and user-friendly platform

Who Can Benefit?
• Students preparing for flow cytometry coursework and exams (i.e., ESCCA European Certificate for Cytometry Operators exam and Specialist in Cytometry, SCYM(ASCP) exam)
• Anyone seeking to enhance and validate their flow cytometry knowledge

Preview

Quiz Sampler

Access

  • Already registered? Click LOGIN