LYNX Rapid & Rapid Plus Conjugation Kits

LYNX Rapid & Rapid Plus Conjugation Kits®

3 Easy Steps & only 30 Seconds Hands-on!

LYNX Rapid & Rapid Plus Conjugation Kits have made the direct labeling of antibodies, proteins and peptides so quick and easy. It only takes 3 simple steps, with 30 seconds hands-on time, to obtain high quality and covalently conjugated antibodies.

  • Fast: One-step labeling procedure. It only requires 30 seconds hands on time, with an incubation time of either just 15 mins with the LYNX Rapid Plus Kits or 3 hours with the LYNX Rapid Kits to finish the conjugation
  • Simple: Can be used in the presence of up to 0.1% of sodium azide and 0.5% BSA
  • Instantly ready-to-use: After the conjugation process is finished there is no additional columns or dialysis required. The stable, high-quality labeled antibodies are ready-to-use
  • 100% Recovery: There is no post-conjugation clean-up required, which means 100% yields of labeled antibodies. LYNX Kits not only save you time, but also eliminate the batch-to-batch variation and the loss of your precious antibodies from using the traditional labeling methods
  • Easy scale up: It is the same procedure for labeling small and large quantity of antibodies. This allows you to initially conjugate micrograms of antibody for trial, and then scale up to milligram quantities
  • Applications: Flow cytometry, ELISA, western blotting, immunohistochemistry and immunofluorescence

3 Easy Steps, only 30 Seconds Hands-on, 100% Yields of Labeled Antibodies!

 
LYNX Rapid Conjugation Kits steps
*LYNX Rapid Plus Kits only

LYNX conjugation kits allow you to label your primary antibody quickly and easily avoiding the use of secondary antibodies with a level of fluorescence you can trust. As can be seen below, where human peripheral blood has been stained, the conjugation kits match the performance of our standard directly conjugated antibodies.

Fig 1. Purified CD4 (MCA1267) and CD8 (MCA1226) were labeled with LNK021RPE and LNK241D650 respectively, and used to stain human peripheral blood.

Fig 1. Purified CD4 (MCA1267) and CD8 (MCA1226) were labeled with LNK021RPE and LNK241D650 respectively, and used to stain human peripheral blood. Alternatively directly labeled antibodies were used. The staining shown is lymphocytes gated on the CD3 positive population. CD4 and CD8 positive T cells can be identified in both plots.

Our full range of LYNX Rapid and Rapid Plus Kits are shown below.

LYNX Conjugation Kits

Excitation (nm) / Emission (nm)* Conjugates LYNX Rapid Plus
Kits**
LYNX Rapid
Kits
No. Vials / Antibody Conjugated
per Vial
493 / 518 Dylight®488 LNK221D488
LNK222D488
LNK223D488
  3 / 200µg
1 / 2mg
3 / 20µg
494 / 520 Fluorescein   LNK061F
LNK062F
LNK063F
3 / 200µg
1 / 2mg
3 / 20µg
550 / 570 Cy3 (Cyanine Dye 3) LNK201CY3
LNK203CY3
  3 / 200µg
3 / 20µg
535 / 575 RPE (R-Phycoerythrin)   LNK021RPE
LNK022RPE
LNK023RPE
LNK024RPE
1 / 60µg
3 / 60µg
1 / 600µg
3 / 10µg
544 / 576 Rhodamine LNK251RHO
LNK253RHO
  3 / 200µg
3 / 20µg
562 / 576 Dylight®550 LNK231D550
LNK233D550
  3 / 200µg
3 / 20µg
535 / 615 RPE/Texas Red®   LNK171PETR
LNK172PETR
LNK173PETR
LNK174PETR
1 / 100µg
3 / 100µg
1 / 1mg
3 / 10µg
650 / 662 APC (Allophycocyanin)   LNK031APC
LNK032APC
LNK033APC
LNK034APC
1 / 150µg
3 / 150µg
1 / 1.5mg
3 / 10µg
643 / 667 Cy5 (Cyanine Dye 5) LNK211CY5
LNK212CY5
  3 / 200µg
1 / 2mg
488 / 670 RPE/Cy5   LNK082C
LNK084C
3 / 60µg
3 / 10µg
652 / 672 Dylight®650 LNK241D650
LNK243D650
  3 / 200µg
3 / 20µg
482 / 677 PerCP   LNK071PERCP
LNK072PERCP
LNK073PERCP
1 / 100µg
3 / 100µg
1 / 1mg
482 / 700 PerCP/Cy5.5   LNK141PERCPCY5.5
LNK142PERCPCY5.5
LNK143PERCPCY5.5
LNK144PERCPCY5.5
1 / 100µg
3 / 100µg
1 / 1mg
3 / 10µg
535 / 694 RPE/Cy5.5   LNK094PECY5.5 3 / 10µg
633 / 776 APC/Cy7   LNK131APCCY7
LNK132APCCY7
LNK133APCCY7
LNK134APCCY7
1 / 150µg
3 / 150µg
1 / 1.5mg
3 / 10µg
535 / 776 RPE/Cy7   LNK111PECY7
LNK112PECY7
LNK113PECY7
LNK114PECY7
1 / 60µg
3 / 60µg
1 / 600µg
3 / 10µg

*Maximum Excitation (nm) / Maximum Emission (nm)
**Only 15 minute incubation time required


Enzymatic Kits

Conjugates LYNX Rapid Kits No. Vials / Antibody Conjugated
per Vial
Alkaline Phosphatase (AP)
(Currently out of stock)
LNK011AP
LNK012AP
LNK013AP
LNK014AP
1 / 100µg
3 / 100µg
1 / 1mg
3 / 10µg
Horseradish Peroxidase (HRP) LNK001P
LNK002P
LNK003P
LNK004P
LNK005P
LNK006P
1 / 400µg
3 / 400µg
1 / 4mg
5 / 4mg
1 / 20mg
3 / 40µg

Biotin and Streptavidin Dyes

Conjugates Lynx Rapid Plus Kits** LYNX Rapid Kits No. Vials / amount conjugated
per vial
Biotin (Type 1) LNK261B
LNK262B
LNK263B


 
3 / 200µg
1 / 2mg
3 / 20µg
Biotin (Type 2) LNK271B
LNK272B
LNK273B


 
3 / 200µg
1 / 2mg
3 / 20µg
Streptavidin   LNK161STR
LNK162STR
LNK163STR
3 / 100µg
1 / 1mg
3 / 10µg

** Only 15 minutes incubation required


 


LYNX Rapid Conjugation Kit® is a registered trademark of Bio-Rad Laboratories, Inc. Cy containing products or portions thereof are manufactured under licence from Carnegie Mellon University under U.S. Patent Number 5,268,486 and related patents. Cy® and CyDye® are trademarks of GE Healthcare Limited. Texas Red® is a registered trademark of Molecular Probes, Inc.  Dylight® is a registered trademark of Thermo Fisher Scientific and its subsidiaries.