BD Biosciences Fluorescence Spectrum Viewer

This online tool is designed to aid customer understanding of important principles, dependencies, and relationships involved in selecting fluorochromes for multicolor flow cytometry. Users of this tool should ultimately rely on their own judgment when selecting fluorochromes for multicolor panels and experiments.

Bandpass filters are specified by central wavelength and Full Width Half Maximum (FWHM), and are rendered by this applet as rectangles. As a result, bandpass filters allow varying amounts light to pass beyond the indicated extents until the blocking is complete. As a result, filter performance estimates from this applet and the actual spillover percentages used on cytometer data may vary. Long pass filters are shown over their emission range.