Beckman Coulter Krome Orange™

Krome Orange™ dye has been optimized for superior optical performance including high signal to noise ratios and minimal intra-laser compensation requirements.

• Narrow, well separated emission profiles
• Excited by violet laser; optimally detected using a 550/40 filter
• Virtually no excitation detected using a 488 nm laser

Krome Orange™ dye is at least as bright as Horizon™ V500 dye and can provide more than twice the population separation obtained with Pacific Orange™ dye conjugates and with little compensation versus Pacific Blue™ dye.

• Less compensation than Horizon V500 dye versus Pacific Blue dye
• Unlike AmCyan, essentially no compensation versus 488-excited channel
• Better population resolution than Pacific Orange™ dye

Krome Orange™ dye is optimally suited to deliver exceptional performance on instruments equipped with a violet laser.

Krome Orange™ dye excitation curve (dashed black line); emission curves for Krome Orange™ dye, Pacific Orange™ dye, AmCyan, and Horizon V500 dye.

CD45 example histogram overlays of the fluorescence excitation and emission spectra showing Krome Orange™ staining patterns relative to other violet laser dyes.

Example histograms showing conjugate staining patterns gated on monocytes except for CD14 overlay showing monocytes and lymphocytes. Histograms are labeled with staining index (SI) values.

*Horizon V500 is a trademark of Becton Dickinson. **Pacific Blue and Pacific Orange are registered trademarks of and licensed under patents assigned to Molecular Probes, Inc.