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- Which Fluorochrome Combination?
DRAQ7™ DNA stain
What is it?
DRAQ7™ is a new far-red fluorescent DNA dye that ONLY stains the nuclei in DEAD and permeabilized cells. It can be combined with other common fluors, especially GFP fusions & FITC and PE labeled antibodies.
It can be excited by the blue, green or red light in a flow cytometer and red light in imaging instruments.
DEAD CELLS / MEMBRANE-COMPROMISED CELLS:
DRAQ7™ does NOT enter intact, live cells. It is excited by blue through red lasers.
DRAQ7™ is NON-TOXIC in long-term culture (see example in SU-DHL4 cells)
DRAQ7™ is an ideal replacement for propidium iodide (and 7-AAD) having far better spectral properties: no UV excitation and no emission overlap with PE and homologues.
DRAQ7™ allows a new design of combination analysis for cell health and status.
DRAQ7™ (viability/integrity) can be directly interchanged with DRAQ5™ (DNA content)
FIXED CELLS & TISSUE SECTIONS:
DRAQ7™ is an ideal far-red DNA counterstain for IF/IHC, high content screening and cell-based assays
What are the key benefits?
DRAQ7™ has many applications and is highly compatible with existing protocols across a wide range of instrumentation platforms.
Key benefits include:-
- Rapid staining of dsDNA/nuclei of DEAD or permeabilized cells
- Combination with live cell dyes for dead/live discrimination
- Easy to use - no wash, no RNase needed
- Ideal for use with GFP & FITC labels - DRAQ7 fluoresces in the far-red region
- Cost-effective analysis
Where can I buy DRAQ7™?
DRAQ7™ can be purchased from this website. See our purchase pages for full details.