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Cytognos Quicklysis™ (Erythrocyte Lysing Solution)
Quicklysis™
Quicklysis™ is an erythrocyte lyse-non-wash method without fixatives that allows the study of leukocyte subpopulations by flow cytometry. It is used in whole blood or bone marrow samples after immunofluorescence staining of the cells, and requires no subsequent washing-centrifugation steps.
The use of Quicklysis™ reduces sample processing time and avoids cell losses associated to the fixation and washing steps, maintaining the relative distribution of the cellular subsets present in the sample. This feature is specially important in absolute counts of CD34+ progenitor cells studies.
References
Menéndez P, et al. Comparison between a lyse-and-then-wash method and a lyse-non-wash technique for the enumeration of CD34+ hematopoietic progenitor cells. Cytometry (Communications in Clinical Cytometry) 34: 264-271 (1998)
Gratama JW, Menéndez P, Kraan J, Orfao A. Loss of CD34+ hematopoietic progenitor cells due to washing can be reduced by the use of fixative-free erytrocyte lysing reagents. J Immunol Methods 26-239: 13-23 (2000)