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EXBIO Apoptosis Assay Kit
Apoptosis Assay Kit - FITC
Apoptosis (a programmed cell death) is a well described phenomenon that occurs in many cellular systems. Currently, the most widely used analytical assays are based on monitoring of translocation of phosphatidylserine (PS) from inner phospholipid layer to the cell surface by use of a fluorochrome-labelled Annexin V in combination with appropriate vital dyes.
The EXBIO Apoptosis Assay Kit is based on a standard setup that employs Annexin V-FITC conjugate and propidium iodide (PI). The flow cytometry test can discriminate intact cells (FITC-/PI-), apoptotic cells (FITC+/PI-) and necrotic cells (FITC+/PI+).
Example
JURKAT cells (human T cell leukemia) were treated with 200 ng/ml TRAIL/Apo2L (bacterially expressed extracellular domain of human TRAIL, corresponding to amino acids 95-281) for 6 hours or left untreated. Detection of apoptotic cells was preformed by flow cytometry using Apoptosis Assay Kit (Cat. No. KIT-ANXF).
A small proportion of untreated cells were identified as undergoing apoptosis (PS expressing, PI negative). In TRAIL-treated cells the number of early apoptotic cells (PS expressing, PI negative) as well as necrotic cells (PS expressing, PI positive cells) significantly increased.
Green line - untreated and unstained cells
Red line - untreated and stained cells
Blue line - TRAIL-treated and stained cells